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Whole genome sequencing of BG2 derived adaptors selected from sub-inhibitory amount of tunicamycin

ABSTRACT: Whole genome sequencing of BG2 derived adaptors selected from sub-inhibitory amount of tunicamycin

PROVIDER: PRJEB58005 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR10558111_1.fastq.gz	Fastqsanger.gz
	ERR10558111_2.fastq.gz	Fastqsanger.gz
	ERR10558112_1.fastq.gz	Fastqsanger.gz
	ERR10558112_2.fastq.gz	Fastqsanger.gz
	ERR10558113_1.fastq.gz	Fastqsanger.gz

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Whole genome sequencing of BG2 derived adaptors selected from sub-inhibitory amount of tunicamycin

Project description:Candida albicans lab strain BG2 was grown in YPD broth supplemented with 0.5ug/ml tunicamycin at 37C for 24h. We used three techinical repeats. The culture was washed, and diluted with distilled water. Then approximately 200 cells from each of the three cultures were spread on YPD plates. Randomly 99 colonies from each culture were tetsted for tolerance to tunicamycin, as compared to the parent BG2. In total we got 6 tolerant adaptors. These 6 adaptors as well as the parent were sequenced.

2022-12-11 | E-MTAB-12427 | biostudies-arrayexpress

Whole genome sequencing of BG2 derived adaptors selected from high amount of tunicamycin

Project description:Approximately 1 million cells of Candida glabrata lab strian BG2 were spread on YPD plate supplemented with 8 ug/ml tunicamycin. Randomly 30 adaptors were chosen. 28 adpators were more tolerant than parent to tunicamycin. These 28 tolerant adaptors, as well as the parent BG2 were sequenced.

2022-12-12 | E-MTAB-12423 | biostudies-arrayexpress

Whole genome sequencing of BG2 derived adaptors selected from high amount of tunicamycin

Project description:Whole genome sequencing of BG2 derived adaptors selected from high amount of tunicamycin

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Candida auris derived tunicamycin adaptors using high amount of tunicamcyin

Project description:Candida auris derived tunicamycin adaptors using high amount of tunicamcyin

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Candida auris derived tunicamycin adaptors using high amount of tunicamcyin

Project description:Candida auris reference strain B11221 was spread on YPD plate supplemented with 8 μg/ml tunicamycin. Randomly 25 adaptors (T2080-T2104) were chosen. These adaptors and the parent were sequenced.

2023-10-05 | E-MTAB-12665 | biostudies-arrayexpress

Whole genome sequencing of sub-MIC tunicamycin adaptors in Cryptococcus neoformans

Project description:Cryptococcus neoformans lab strain H99 was grown in YPD broth supplemented with 0.0625ug/ml tunicamycin. We did three technical replicates. Randomly 120 colones from each culture were tested for tolerance to tunicamycin. Totally we got 7 tolerant adaptors. These adaptors were sequenced.

2023-07-13 | E-MTAB-12268 | biostudies-arrayexpress

Whole genome sequencing of adaptors selected by sub-inhibitory amount of brefeldin A in Candida albicans

Project description:In Candida albicans, the lab strain SC5314 was grown in YPD broth supplemented with 4ug/ml brefeldin A for 24h. The culture was washed, diluted and spread on YPD. Randomly 120 colonies were tested for tolerance to brefeldin A. Two adaptors (FY200 and FY201) obtained tolerance. These two adaptors were sequenced.

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Whole genome sequencing of adaptors selected by sub-inhibitory amount of brefeldin A in Candida albicans

Project description:Whole genome sequencing of adaptors selected by sub-inhibitory amount of brefeldin A in Candida albicans

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Whole genome sequencing of Candida albicans tunicamycin adaptors

Project description:We used Candida albicans lab strain SC5314 to obtain tunicamycin adaptors. We did whole genome sequencing of the adaptors and the parent as well.

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Whole genome sequencing of tunicamycin adaptors in Candida parapsilosis

Project description:We exposed Candida parapsilosis clinical isolate #12108 to YPD plate supplemented with 8µg/ml of tunicamycin. We randomly selected 18 adaptors. We did sequencing of these adaptors.

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