Project description:Cappable-seq was used to map transcription start sites globally in Salmonella Typhimurium SL1344. In addition to naturally occurring plasmids pSLT and pCol1B9, the cells carry plasmid pAMNF. The latter can be used for low level constitutive expression of a transcription factor of interest, though in these experiments no such ectopic transcription factor expression was used.
Project description:Cappable-seq was used to map transcription start sites globally in Salmonella Typhimurium SL1344. In addition to naturally occurring plasmids pSLT and pCol1B9, the cells carry derivatives of plasmid pAMNF, or pAMNM, that drive low level constitutive expression of epitope tagged MarA, SoxS, Rob or RamA derivatives.
Project description:We performed Chromatin Immunoprecipitation (ChIP) and microarray hybridization analysis of CspC binding in Salmonella Typhimurium strain SL1344 which has been genetically engineered to express a 3xFLAG tagged CspC protein.
Project description:We performed transcriptome abundance analysis of Salmonella Typhimurium strain SL1344 swap which has been genetically engineered to express the hns open-reading frame from the stpA promoter and the stpA open reading frame from the hns promoter. This strain is designated SL1344(swap). Transcript abundance was compared with that of wild-type SL1344. This comparison was performed to determine the effect of chromosome location of the expression of two related global regulators and how alterations to their expression patterns would impact on their regulons.
Project description:FabR ChIP-chip on Salmonella enterica subsp. enterica serovar Typhimurium SL1344 using anti-Myc antibody against strain with chromosomally 9Myc-tagged FabR (IP samples) and wildtype strain (mock IP samples)