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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling by array of Salmonella enterica serovar Typhimurium SL1344 and a SL1344 soxS knockout

ABSTRACT: The transcriptome of Salmonella enterica serovar Typhimurium SL1344 was compared to that of SL1344 with soxS knocked out.

ORGANISM(S): Salmonella typhimurium

SUBMITTER: Andrew Bailey

PROVIDER: E-MEXP-2888 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcription profiling by array of Salmonella enterica serovar Typhimurium SL1344 and a SL1344 marA knockout

Project description:The transcriptome of Salmonella enterica serovar Typhimurium SL1344 was compared to an isogenic mutant in which marA was deleted.

2010-12-01 | E-MEXP-2886 | biostudies-arrayexpress

Minimal vs LB media comparison

Project description:The type of bacterial culture medium is an important consideration during design of any experimental protocol. The aim of this study was to understand the impact of medium choice on bacterial gene expression and physiology by comparing the transcriptome of Salmonella enterica SL1344 after growth in the widely used LB broth or the rationally designed MOPS minimal medium. Transcriptomics showed that after growth in MOPS minimal media, compared to LB, there was increased expression of 42 genes involved in amino acid synthesis and 23 genes coding for ABC transporters. Seven flagellar genes had decreased expression after growth in MOPS minimal medium and this correlated with a decreased motility. In both MOPS minimal medium and MEM expression of genes from SPI-2 was increased and the adhesion of S. Typhimurium to intestinal epithelial cells was higher compared to the levels after growth in LB. However, SL1344 invasion was not significantly altered by growth in either MOPs minimal media or MEM. Expression of SPI-2 was also measured using chromosomal GFP reporter fusions followed by flow cytometry which showed, for the first time, that the reduction in SPI-2 transcript after growth in different media related to a reduction in the proportion of the bacterial population expressing SPI-2. These data highlight the profound differences in the global transcriptome after in vitro growth in different media and show that choice of medium should be considered carefully during experimental design, particularly when virulence related phenotypes are being measured.

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Project description:S. Typhimurium SL1344 was exposed to the minimum inhibitory concentration of triclosan and the transcriptomic response analysed.

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