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PROVIDER: PRJEB59233 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Expression response of cells stimulated with Y. pestis, including genes which overlap candidates for postiive selection inferred using ancient genomes.
2022-08-17 | GSE194118 | GEO
Project description:288 strains from a worldwide collection of Yersinia pestis were genotyped. The SNP positions tested were from a comparison of 15 genomes plus low resolution SNP discovery in up to 186 kb with dHPLC.
2010-05-01 | E-MTAB-213 | biostudies-arrayexpress
Project description:Human mesenchymal stem cells are a promising cell source for the treatment of stroke. Their primary mechanism of action occurs via neuroprotective effects by trophic factors, anti-inflammatory effects, and immunomodulation. However, the regeneration of damaged neuronal networks by cell transplantation remains still challenging. We hypothesized that cells induced to neural lineages would fit the niche, replace the lesion, and be more effective in improving symptoms compared with stem cells themselves. We investigated the characteristics of induced neural cells from human dental pulp tissue and compared the transplantation effects between these induced neural cells and uninduced dental pulp stem cells. Induced neural cells or dental pulp stem cells were intracerebrally transplanted 5 days after cerebral infarction induced by permanent middle cerebral artery occlusion in immunodeficient mice. Effects on functional recovery were also assessed through behavior testing. We used immunohistochemistry and neuron tracing to analyze the differentiation, axonal extension, and connectivity of transplanted cells to the host’s neural circuit. Transplantation of induced neural cells from human dental pulp ameliorated functional recovery after cerebral infarction compared with dental pulp stem cells. The induced neural cells comprised both neurons and glia and expressed functional voltage, and they were more related to neurogenesis in terms of transcriptomics. Induced neural cells had a higher viability than did dental pulp stem cells in hypoxic culture. We showed that induced neural cells from dental pulp tissue offer a novel therapeutic approach for recovery after cerebral infarction.
2021-09-01 | GSE174260 | GEO
Project description:Metabolomic approaches, such as in clinical applications of living individuals, have shown potential use for solving questions regarding the past when applied to archaeological material. Here, we study for the first time the potential of this Omic approach as applied to metabolites extracted from archaeological human dentin. Dentin obtained from micro sampling the dental pulp of teeth of victims and non-victims of <i>Yersinia pestis</i> (plague) from a 6th century Cambridgeshire site are used to evaluate the potential use of such unique material for untargeted metabolomic studies on disease state through liquid chromatography hyphenated to high-resolution mass spectrometry (LC-HRMS). Results show that small molecules of both likely endogenous and exogenous sources are preserved for a range of polar and less polar/apolar metabolites in archaeological dentin; however, untargeted metabolomic profiles show no clear differentiation between healthy and infected individuals in the small sample analysed (<i>n</i> = 20). This study discusses the potential of dentin as a source of small molecules for metabolomic assays and highlights: (1) the need for follow up research to optimise sampling protocols, (2) the requirements of studies with larger sample numbers and (3) the necessity of more databases to amplify the positive results achievable with this Omic technique in the archaeological sciences.
2024-03-15 | MTBLS7394 | MetaboLights
Project description:Ancient Y. pestis genomes lack the virulence-associated YpfΦ prophage present in Modern pandemic strains
| PRJEB60595 | ENA
Project description:Using the HumanMethylation450 Beadchip, whole genomes of human dental pulp stem cells (DPSCs), periodontal ligament stem cells (PDLSCs), and dental follicle progenitor cells (DFPCs) were compared.The DNA methylation profiles were obtained across approximately 485,512 CpGs in human odontogenic stem cells samples. Samples included DPSCs, DFPCs and PDLSCs from each 4 (12 in total) human individuals.
2021-04-10 | GSE112933 | GEO
Project description:Ancient Yersinia pestis and Salmonella enterica genomes from Bronze Age Crete
| PRJEB52494 | ENA
Project description:We looked at binding sites in wild type Yersinia pestis using a strain with psaE either FLAG-tagged or His-tagged.
2019-10-04 | E-MTAB-8370 | biostudies-arrayexpress
Project description:Methanobrevibacter massiliense and Pyramidobacter piscolens co-culture from infected dental pulp
| PRJEB63538 | ENA