Interrogating the gut microbiota from several human-biting tick species in Northwestern Spain
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PROVIDER: PRJEB59487 | ENA |
REPOSITORIES: ENA
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Project description:This study measured the differential expression of genes from two populations of Wyeomyia smithii in the USA that differ in their propensity to bite over a geographic gradient. Population KC is a northern non-biting population from Maine; population WI is a southern low-frequency biting population from Florida. The mosquitoes were maintained at the University of Oregon under standard rearing conditions and run through two generations to minimize maternal and field effects. Selection for biting was conducted using ~14,000 individuals from the WI population. The environment and protocols used for selection were the same as for KC except biters were removed from their cage and placed into a separate “biting” cage with supplemental males from the same generation of the selected line. All hatch from the biting cage were used to generate the subsequent generations. This protocol was followed until the selected line had undergone 5 generation of selection. Through all generations of selection, including those that were not offered a host, hatch were placed on short days (LL:D=10:14) at 21°C to synchronize each generation and to miigate inadvertant direct selection on development time, generation time, or the timing of reproductive allocation. After adults of a given generation had died, their offspring were transferred to long days and reared to adulthood, as above. During experiments, all animals were in good physical condition. Animal heads were dissected then transferred into 1.5-ml microcentrifuge tubes and shock-frozen in liquid nitrogen. Samples were short-term stored at -80°C. For all experimental conditions, four independent replicates (50 heads each) were analyzed. This GEO record is for three comparisons among the three populations: (EXP1) non-biters in the base population (WI) versus biting individuals in the WI line selected for biting (WIOB) over 5 generations of selection (WI vs WIOB); (EXP2) WIOB versus northern obligate non-biters (KC); (3) WI versus KC (n=4 per treatment group for each population).
2017-12-31 | GSE100766 | GEO
Project description:Gene expression in the hypothalamus and pre frontal cortex in pigs performing and receiving tail biting behaviour was compared with expression in pigs not performing or receiving tail biting
2013-11-12 | E-MEXP-3630 | biostudies-arrayexpress
Project description:Brain gene expression in pigs housed in a pen during a tail biting outbreak compared with pigs housed in control pens.
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Project description:The biting behavior observed in Carpenter ants infected by the specialized fungus Ophiocordyceps unilateralis s.l. is an example of a complex host behavioral manipulation by parasite. Though parasitic manipulation of host behavior is generally assumed to be due to the parasite’s gene expression, few studies have set out to test this. We experimentally infected Carpenter ants to collect tissue from both parasite and host during the time period when manipulated biting behavior is experienced. Upon observation of synchronized biting, samples were collected and subjected to RNA-Seq analyses. We also sequenced and annotated the O. unilateralis s.l. genome as a reference for the fungal reads. Our mixed transcriptomics approach, together with a comparative genomics study, shows that the majority of the fungal genes that are up-regulated during manipulated biting behavior are unique to the O. unilateralis s.l. genome. This study furthermore reveals that the fungal parasite might be regulating immune- and neuronal stress responses in the host during manipulated biting, as well as impairing its chemosensory communication and causing apoptosis. Moreover, we found genes up-regulated during manipulation that putatively encode for proteins with reported effects on behavioral outputs, proteins involved in various neuropathologies, and proteins involved in the biosynthesis of secondary metabolites such as alkaloids.
2015-08-18 | GSE68176 | GEO
Project description:This experiment was undertaken to document changes in gene expression in the skin of tick-resistant Brahman (Bos indicus) and tick-susceptible Holstein-Friesian (Bos taurus) cattle prior to, and following, infestation with the cattle tick Rhipicephalus (Boophilus) microplus Experiment Overall Design: RNA was extracted from skin samples of tick-naïve cattle (animals with no previous R.microplus exposure) and tick-infested cattle after a period of successive, heavy infestations with R. microplus. Skin samples taken from tick-infested animals were taken at sites where tick larvae (approximately 24 h old) were attached to the skin sample. Skin samples were of 8 mm diameter and full skin thickness (approximately 10 mm). RNA samples from 12 animals (3 tick-naive Holstein-Friesian, 3 tick-naive Brahman, 3 tick-infested Holstein-Friesian and 3 tick-infested Brahman) were processed and hybridised to individual slides.
2009-04-11 | E-GEOD-15342 | biostudies-arrayexpress
Project description:Ixodes species ticks are competent vectors of tick-borne viruses including tick-borne encephalitis and Powassan encephalitis. Tick saliva has been shown to facilitate and enhance viral infection. This likely occurs by saliva-mediated modulation of host responses into patterns favorable for viral infection and dissemination. Because of the rapid kinetics of tick-borne viral transmission, this modulation must occur as early as tick attachment and initiation of feeding. In this study, the gene expression profile of cutaneous bite-site lesions created by uninfected ticks were analyzed at 1, 3, 6, and 12 hours after Ixodes scapularis nymphal tick attachment to discover host pathways or responses potentially important in tick-borne viral establishment.
2012-12-21 | GSE39100 | GEO
Project description:eMERGE: Northwestern (NUgene) WGS
| PRJNA339189 | ENA
Project description:This experiment was undertaken to document changes in gene expression in the skin of tick-resistant Brahman (Bos indicus) and tick-susceptible Holstein-Friesian (Bos taurus) cattle prior to, and following, infestation with the cattle tick Rhipicephalus (Boophilus) microplus Keywords: Disease state analysis
2009-04-03 | GSE15342 | GEO
Project description:Ixodes species ticks are competent vectors of tick-borne viruses including tick-borne encephalitis and Powassan encephalitis. Tick saliva has been shown to facilitate and enhance viral infection. This likely occurs by saliva-mediated modulation of host responses into patterns favorable for viral infection and dissemination. Because of the rapid kinetics of tick-borne viral transmission, this modulation must occur as early as tick attachment and initiation of feeding. In this study, the gene expression profile of cutaneous bite-site lesions created by uninfected ticks were analyzed at 1, 3, 6, and 12 hours after Ixodes scapularis nymphal tick attachment to discover host pathways or responses potentially important in tick-borne viral establishment. Four milimeter ear biopsies from BALB/cJ mice infested with Ixodes scapularis nymphs were assayed using Affymetrix genechip 430A 2.0 arrays at 1, 3, 6, and 12 hours after infestation during a primary exposure. 3 mice were measured at each time point. Controls were 3 similarly housed but tick-free mice.
2012-12-21 | E-GEOD-39100 | biostudies-arrayexpress