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ScATAC-seq of mouse liver tissue derived from young and old mice II

ABSTRACT: scATAC-seq of mouse liver tissue derived from young and old mice II

PROVIDER: PRJEB59590 | ENA |

REPOSITORIES: ENA

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scATAC-seq of mouse liver tissue derived from young and old mice II

Project description:We investigate if the differences in phenotype and transcriptome over age might be explained by an underlying change on the epigenetic level. We performed single-cell ATAC sequencing using the 10x Chromium platform. We profiled 2259 nuclei prepared from 3 young liver tissues and 2490 nuclei from 3 old liver tissues.

2023-11-09 | E-MTAB-12560 | biostudies-arrayexpress

scATAC-seq of mouse liver tissue derived from young and old mice

Project description:scATAC-seq of mouse liver tissue derived from young and old mice

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scATAC-seq of mouse liver tissue derived from young and old mice

Project description:We investigate if the differences in phenotype and transcriptome over age might be explained by an underlying change on the epigenetic level. We performed single-cell ATAC sequencing using the 10x Chromium platform. We profiled 4838 nuclei prepared from 3 young liver tissues and 3361 nuclei from 3 old liver tissues.

2023-02-20 | E-MTAB-12706 | biostudies-arrayexpress

ATACseq for Young and Old mouse liver

Project description:ATACseq for Young and Old mouse liver

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mRNA expression of young, mid-old, and old fibroblasts

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TMT isobaric profiling of young versus old haematopoietic stem cells

Project description:Mass spectrometry was performed with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific) interfaced with an UltiMate 3000 Binary RSLCnano System (Dionex). Proteome Discoverer v.1.4 (Thermo Scientific) with SEQUEST HT search engines was used for the spectra-preprocessing and HCD MS2 spectra were used for peptide identification and quantitation based on TMT reporter ions. TMT isobaric comparison of old versus young haematopoietic stem and progenitor cells. Young 1 and Young 2 are samples 126 and 128 of dataset UTH_1. Old 1 and Old 2 are samples 129 and 130 of UTH_1. Young 3 is sample 131 and Old 3 is sample 130 of dataset UTH_4.

2023-10-16 | MSV000093126 | MassIVE

mRNA expression of young, mid-old, and old fibroblasts

Project description:Examination of the difference in mRNA expression profile between young, mid-old, and old fibroblasts

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scRNA-seq of mouse liver tissue derived from young and old mice

Project description:scRNA-seq of mouse liver tissue derived from young and old mice

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RNA sequencing of young and old lung ILC2 cells

Project description:ILC2 cells from young and old mouse lungs were analysed and revealed altered transcriptome of old ILC2 compared to the young.

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scRNA-seq of mouse liver tissue derived from young and old mice

Project description:We want to investigate how cells in the specific zones in murine liver are affected by age-related changes of the microenvironment. To this end, we generated high-quality scRNA-seq dataset of hepatocytes using Smart-seq3express from 2 young (3-5 months) and 2 old (18-20 months) male mice. Livers were perfused and viable hepatocytes were FACS-sorted based on size. In addition, we recorded ploidy levels of hepatocytes. We retained 545 hepatocytes in total after initial filtering.

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