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Sequencing of porcine cell line PK15 and porcine fibroblasts

ABSTRACT: Sequencing of porcine cell line PK15 and porcine fibroblasts

PROVIDER: PRJEB60951 | ENA |

REPOSITORIES: ENA

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MeRIP sequencing analysis of PK15 cell line infected with PRV

Project description:Purpose: Evaluation of the m6A modification of PRV and PK15 transcripts during PRV infection Methods: Porcine kidney cell line PK15 was uninfected or infected with PRV for 24 hours. Total RNA from each sample were extracted. Intact mRNA was isolated from total RNA samples and then chemically fragmented to 300-nucleoside-long fragments. Fragmented mRNAs were immunoprecipitated with anti-N6-methyadenosine (m6A) antibody (a part of the fragmented mRNAs was kept as input). Both m6A enriched mRNAs and input mRNAs were concentrated for RNA-seq libraries construction. The libraries were forwarded to sequencing run on Illumina NovaSeq 6000. Results: PRV transcripts were m6A modified during PRV infection and PRV infection changed m6A modification profiles of PK15 transcripts.

2022-11-02 | GSE209949 | GEO

Expression data from porcine kidney cell (PK15) transfected by the plasmids

Project description:Several recombinat viruses of porcine circovirus type 2 (PCV2),including P1, P2, ZJ-R, VL258, and VL264, have been found. The PK15 cells were transfected by the molecular clones of the abovementioned viruses, where specific sets of genes are up-regulated or down-regulated. We used microarrays to detail the global programme of gene expression and identified distinct functions of viruses or viral proteins. PK15 cells were selected at 12 hours post-transfection for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain the gene expression patterns of PK15 cells transfected with different molecular clones of the viruses.

2015-08-12 | E-GEOD-71945 | biostudies-arrayexpress

Transcription profiling of porcine olfactory bulb neuroblasts and PK15 non-neuronal, epithelial origin cell cultures

Project description:The phenotypically characterized hTERT immortalized porcine olfactory bulb neuroblast cell line (OBGF400) was subjected to an extensive whole genome-scaled expression profile for establishing their use as an in vitro neuronal disease model system. Microarrays were used to provide a comprehensive knowledge underlying the genomic complexity and overall gene expression capacity of the immortalized OBGF400 cells. The analysis revealed the elaborate signaling mechanisms of this unique subpopulation of porcine neuronally committed progenitors that mirrors the intricate organization of postnatal neurongenic zones. SUBMITTER_CITATION: Transcriptome Profile and Cytogenetic Analysis of Immortalized Neuronally Restricted Progenitor Cells Derived from the Porcine Olfactory Bulb. Animal biotechnology 2009 vol:20 iss:4 page:186-215 Experiment Overall Design: Total cellular RNA extracts from independent OBGF400 (neuroblasts) and PK15 (non-neuronal, epithelial origin) cell cultures (9 each) were pooled into a total of 3 biological replicates per cell line. The concentrations and purity of the pooled RNA preparations were determined using the BioAgilent RNA assay prior to hybridization on Affymetrix GeneChipÂ® Porcine Genome Expression Arrays. To ascertain the genes that were preferentially expressed by the OBGF400 neuroblasts, we used the PK15 cellular array in an effort to exclude somatic cell background.

2009-11-10 | E-GEOD-16855 | biostudies-arrayexpress

Expression data from porcine kidney cell (PK15) transfected by the plasmids

2015-08-12 | GSE71945 | GEO

MeRIP sequencing analysis of PK15 cell line infected with PRV

Project description:MeRIP sequencing analysis of PK15 cell line infected with PRV

| PRJNA863143 | ENA

Transcriptome sequencing of PCV2 ORF5 overexpressed PK15 cells

Project description:This study investigated the immunological function of PCV2 ORF5 by ectopic expression of PCV2 ORF5 in PK15 cell line. Identifying the functional role of each PCV2 ORF associated with host cell modulation may provide better knowledge about the pathogenesis of postweaning multisystemic wasting syndrome (PMWS). PCV2 ORF5 is recently identified and the functional role of ORF5 during the pathogenesis after PCV2 infection is largely unknown.

2021-03-04 | GSE111411 | GEO

Changes in cellular microRNA expression induced by porcine circovirus type 2-encoded proteins.

Project description:We employed deep sequencing technology to uncover cellular miRNAs differentially regulated after expression of each of three PCV2-encoded open reading frames (ORFs) in porcine kidney epithelial PK15 cells. Control PK15 vs. PCV2 ORF1, ORF2, ORF3-expressing PK15.

2014-08-08 | E-GEOD-60206 | biostudies-arrayexpress

PrV/PK15 kinetics_QiagenNRSP8 slides

Project description:To study in vitro the epithelial cells and PrV interactions during infection, we followed PrV and PK15 cells transcriptome modifications during time-course infection (I) and mock-infection (MI). Four time points were studied: 1h, 2h, 4h and 8h post-I and MI. Four replicates of I and MI were analysed. Keywords: Pig, PrV, Pk15 cells, kinetics

2007-12-01 | GSE9259 | GEO

Changes in cellular microRNA expression induced by porcine circovirus type 2-encoded proteins.

2014-08-08 | GSE60206 | GEO

Comparison of the proteomes of porcine macrophages and a stable porcine cell line after infection with African swine fever virus

Project description:Quantitiative analysis of proteomes of porcine macrophages and the stable cell line WSL infected with African swine fever virus using a nanoLC MALDI-Tof/Tof MS platform. The impact of the infection on the two cell types was analyzed using Gene Ontology term and KEGG pathway enrichment analysis.

2021-10-26 | PXD028838 | JPOST Repository

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