Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

In trans adaptation and RNA-guided silencing by a Klebsiella plasmid-encoded type IV-A3 CRISPR-Cas system

ABSTRACT: Functional characterization of a plasmid-encoded type IV-A3 CRISPR-Cas system

PROVIDER: PRJEB61733 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Structural variation of types IV-A1- and IV-A3-mediated CRISPR interference

Project description:CRISPR-Cas mediated DNA-interference typically relies on sequence-specific binding and nucleolytic degradation of foreign genetic material. Type IV-A CRISPR-Cas systems diverge from this general mechanism, using a nuclease-independent interference pathway to suppress gene expression for gene regulation and plasmid competition. To understand how the type IV-A system associated effector complex achieves this interference, we determined cryo-EM structures of two evolutionarily distinct type IV-A complexes (types IV-38 A1 and IV-A3) bound to cognate DNA-targets in the presence and absence of the type IV-A signature DinG effector helicase. The structures reveal how the effector complexes recognize the protospacer adjacent motif and target-strand DNA to form an R-loop structure. Additionally, we reveal differences between types IV-A1 and IV-A3 in DNA interactions and structural motifs that allow for in trans recruitment of DinG. Our study provides a detailed view of type IV-A mediated DNA-interference and presents a structural foundation for engineering type IV-A-based genome editing tools.

2024-10-08 | PXD056399 | Pride

A Type IV-A CRISPR-Cas System in Pseudomonas aeruginosa Mediates RNA-Guided Plasmid Interference In Vivo.

Project description:Bacteria and archaea use CRISPR-Cas adaptive immune systems to destroy complementary nucleic acids using RNAs derived from CRISPR loci. Here, we provide the first functional evidence for type IV CRISPR-Cas, demonstrating that the system from Pseudomonas aeruginosa strain PA83 mediates RNA-guided interference against a plasmid in vivo, both clearing the plasmid and inhibiting its uptake. This interference depends on the putative NTP-dependent helicase activity of Csf4/DinG.

| S-EPMC6919247 | biostudies-literature

Deep sequencing data from PAM screen of Pa83 type IV-A CRISPR-Cas system

Project description:In this work the favorable PAM sequences for a type IV-A CRISPR-Cas system were determined

2022-08-19 | GSE211446 | GEO

Aeromonas sp. A3 strain:A3

Project description:Aeromonas sp. A3 strain:A3 Genome sequencing

| PRJNA1135467 | ENA

Aquamicrobium sp. A3 strain:A3

Project description:Aquamicrobium sp. A3 strain:A3 Genome sequencing

| PRJNA869129 | ENA

Salmonella Cascade assembles on both cis- and -trans encoded CRISPR RNAs

Project description:CRISPR/Cas loci commonly encode both proteins and small guide RNAs (crRNAs) to assemble ribonucleoproteins particles (RNPs) that confer a sequence-based, adaptive immunity against viruses and plasmids in prokaryotes. However, it has not been established whether this conserved synteny of RNA and protein genes is needed for the efficient RNP production in vivo. We show that the pathogenic bacterium Salmonella Typhimurium harbours two physically unlinked loci, CRISPR01 and CRISPR02, both of which produce mature crRNAs, although CRISPR02 lacks the protein genes for the type I-specific Cascade complex. Utilizing these coexisting complete and orphan CRISPR loci, we provide the first in vivo evidence for a crRNA-dependent assembly of Cascade. Strikingly, the full RNP assembles identically on crRNAs from either CRISPR cassette, i.e. regardless of their expression in cis or trans. Deep sequencing of Cas-bound transcripts identifies crRNAs derived from both loci as the sole bona fide targets of Cascade, although individual Cas proteins may interact with other cellular RNAs outside of the Cascade context. Altogether, our data demonstrate that CRISPR RNAs, independently of their origin, serve as the organizing centre of Cascade in vivo and are the main reason-to-be for its protein components in Salmonella.

2017-04-24 | GSE58602 | GEO

Aquabacterium sp. A3 strain:A3

Project description:Aquabacterium sp. A3 strain:A3 Genome sequencing and assembly

| PRJNA1093217 | ENA

Pseudoalteromonas sp. A3 strain:A3

Project description:Pseudoalteromonas sp. A3 strain:A3 Genome sequencing and assembly

| PRJNA892777 | ENA

Streptomyces coelicolor A3(2) Timecourse Proteomics

Project description:Streptomyces coelicolor A3(2) shotgun proteomics. S. coelicolor A3(2) was fermented in GYM.

2022-03-23 | MSV000089111 | MassIVE

Citrobacter braakii strain:A3 | isolate:A3

Project description:Citrobacter braakii strain:A3 | isolate:A3 Genome sequencing and assembly