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3' UTR RNA-seq of EGFR-mutant PC9 cells and drug-resistant clones

ABSTRACT: 3' UTR RNA-seq of EGFR-mutant PC9 cells and drug-resistant clones

PROVIDER: PRJEB62844 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR11523483.fastq.gz	Fastqsanger.gz
	ERR11523484.fastq.gz	Fastqsanger.gz
	ERR11523485.fastq.gz	Fastqsanger.gz
	ERR11523486.fastq.gz	Fastqsanger.gz
	ERR11523487.fastq.gz	Fastqsanger.gz

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3' UTR RNA-seq of EGFR-mutant PC9 cells and drug-resistant clones

Project description:EGFR-mutant lung adenocarcnioma cell line PC9 pools were treated with different compounds until resistant cells regrew. Resistant and parental cells were then sequenced to study transcriptional changes.

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Project description:Given the recent reports on the role of AXL in mediating resistance to EGFR-targeted therapy, we generated cell line models of Erlotinib-resistance to investigate the effect of AXL inhibitors on EGFR TKI resistance. For this, EGFR-mutant PC9 cells were passed through a persister bottleneck by applying strong drug selection pressure to generate drug-tolerant erlotinib persister cells. We created four Erlotinib-resistant clones from one parental population; S1-34, S2-10, S2-17 and S2-30. Whole exome and RNA sequencing analyses were performed to probe the differences in Erlotinib-resistance mechanisms present in these persister-derived Erlotinib-resistant cells.

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