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ChIP-seq H3K4me3 on sperm from FVB/NCrl-Trim66em2(gfp)Emr

ABSTRACT: ChIP-seq H3K4me3 on sperm from FVB/NCrl-Trim66em2(gfp)Emr

PROVIDER: PRJEB63269 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR11573862.fastq.gz	Fastqsanger.gz
	ERR11573863.fastq.gz	Fastqsanger.gz
	ERR11573864.fastq.gz	Fastqsanger.gz
	ERR11573865.fastq.gz	Fastqsanger.gz
	ERR11573866.fastq.gz	Fastqsanger.gz

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ChIP-seq H3K4me3 on sperm from FVB/NCrl-Trim66em2(gfp)Emr

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Project description:Total RNA-seq on round spermatids from FVB/NCrl-Trim66em2(gfp)Emr mice

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Next generation sequencing facilitates quantitative analysis of Caenorhabditis elegans N2 wild type, emr-1(gk119), lem-2(tm1582) and emr-1(RNAi) lem-2(tm1582) transcriptomes.

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DamID of Dam::LMN-1 and Dam::EMR-1 in Caenorhabditis elegans adult worms

Project description:Laminopathies are caused by mutations in components of the nuclear envelope (NE). While most NE components are widely expressed, laminopathies affect only a subset of tissues. However, the understanding of the molecular mechanisms that explain this phenomenon is still elusive. Here we have performed a genome wide DamID analysis in adult C. elegans nematodes comparing the DNA association profile of two components of the NE, Lamin/LMN-1 and Emerin/EMR-1. Although both proteins were associated to silent DNA, EMR-1 showed a predominant role in the anchoring of muscle and neuronal promoters to the nuclear periphery. Deletion of either EMR-1 or LEM-2, another integral NE protein, caused local changes in nuclear architecture with both increased and decreased LMN-1 association.

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