Project description:We performed 10x single cell RNA and VDJ sequencing on sorted T cells (7AAD- Calcein blue+ CD45+ THY1.1- TCRb+) in an orthotopic EMT6 tumor model 7 days after treatment initiation in four experimental groups: 1) Control 2) aPD-L1 3) aTGFb 4) aPD-L1 and aTGFb.
Project description:We performed bulk RNA sequencing on sorted T cells (7AAD- Calcein blue+ CD45+ THY1.1- TCRb+) in an orthotopic EMT6 tumor model 7 days after treatment initiation in four experimental groups: 1) Control 2) aPD-L1 3) aTGFb 4) aPD-L1 and aTGFb.
Project description:We performed 10x single cell RNA-seq on sorted populations: T cell (7AAD- Calcein blue+ CD45+ THY1.1- TCRb+), fibroblasts (7AAD- Calcein Blue+ CD45- THY1.1- CD31- PDPN+), myeloid cells (7AAD- Calcein Blue+ CD45+ THY1.1- CD11b+) and tumor cells (7AAD- Calcein Blue+ CD45- THY1.1+) from cells in an orthotopic EMT6 tumor model 7 days after treatment initiation in four experimental groups: 1) Control 2) aPD-L1 3) aTGFb 4) aPD-L1 and aTGFb.
Project description:RNA sequencing analysis was used to identify changes in mouse breast cancer (EMT6) following prolonged anti-PD-L1 in vitro (PDRvitro).
Project description:Mouse EMT6 breast cancer cells were grown in 3D Matrigel culture. Effects of the glycosaminoglycan chondroitin sulfate-E (CS-E) versus untreated controls on transcriptional programs was investigated.
Project description:Transcriptome analyses of isotype-treated and aPD-L1-treated CD4+ T-cells, PD1- Treg cells and PD1+ Treg cells from a mouse model for mesothelioma.