Project description:The purpose of this study is to identify lncRNAs involved in the pathology of colorectal cancer (CRC) progression and investigate their underlying mechanisms. The differentially expressed lncRNAs were identified between 15 CRC tissues and 15 adjacent normal tissues by Arraystar lncRNA microarrays
Project description:Genome wide DNA methylation profiling of peripheral blood mononuclear cells(PBMCs) in normal and CRC samples. The Illumina Infinium 850k Human DNA methylation Beadchip was used to obtain DNA methylation profiles across approximately 820,000 CpGs in PBMC samples. Samples included 50 newly diagnosed CRC patients and 50 normal controls. NOTE:Patients numbered "CRC" and "E-CRC" are from two wards of the same hospital and do not need to be treated differently in data analysis.
Project description:HILEC from CRC tissues isolated from human patients underwent RNA sequencing to define their expression profile by comparison with healthy cells.
Project description:We performed Next Generation Sequencing of normal human intestinal epithelial cell lines, CRC cell lines and CRC cell lines with hypoxia treatment to identify the differentially expressed non-coding RNAs.
Project description:CRC tissues plus matched normal tissues (Primary colon cancer, liver metastasis colon cancer and matched normal colon tissues from same patient) were subjected to RNA isolation and RNA-seq analyses were generated by deep sequencing using Illumina Hiseq.
Project description:The challenge of preventing colorectal cancer (CRC) is the early identification of individuals whose apparently normal colorectal mucosa will develop cancer, because of inherited trait or environmental exposure. We sought to use genome-wide expression profiling of endoscopic biopsies to detect a signature of propensity for cancer. We performed oligonucleotide microarray analysis of normal appearing mucosa of the following cases: healthy individuals, disease-free carriers predisposed to HNPCC (hereditary non-polyposis CRC), disease-free patients who underwent curative large bowel resection for CRC 1 to 15 years earlier and patients with CRC. This profiling is based on the analysis of 4 donors who underwent curative large bowel resection for CRC from 1 to 19 years before (M-CRC samples), 4 disease-free carriers of mutations in the mismatch repair system genes, who are predisposed to develop HNPCC (HNPCC samples) and 4 endoscopy-negative, asymptomatic individuals (NOR samples)
Project description:The challenge of preventing colorectal cancer (CRC) is the early identification of individuals whose apparently normal colorectal mucosa will develop cancer, because of inherited trait or environmental exposure. We sought to use genome-wide expression profiling of endoscopic biopsies to detect a signature of propensity for cancer. We performed oligonucleotide microarray analysis of normal appearing mucosa of the following cases: healthy individuals, disease-free carriers predisposed to HNPCC (hereditary non-polyposis CRC), disease-free patients who underwent curative large bowel resection for CRC 1 to 15 years earlier and patients with CRC.
