Iron acquisition by a commensal bacterium modifies host nutritional immunity during Salmonella infection
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB63649 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| ERR11635627_1.fastq.gz | Fastqsanger.gz | |||
| ERR11635627_2.fastq.gz | Fastqsanger.gz | |||
| ERR11635628_1.fastq.gz | Fastqsanger.gz | |||
| ERR11635628_2.fastq.gz | Fastqsanger.gz | |||
| ERR11635629_1.fastq.gz | Fastqsanger.gz |
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Project description:Proteus mirabilis is a primary cause of complicated urinary tract infections (UTI). Surprisingly, iron acquisition systems have been poorly characterized in this uropathogen despite the urinary tract being iron-limited. In this report the transcriptome of strain HI4320, cultured under iron limitation, was examined using microarray analysis. Of genes upregulated at least 2-fold, 45 were statistically significant and comprise 21 putative iron-regulated systems. Two of these systems, PMI0229-0239 and PMI2596-2605, are organized in operons and appear to encode siderophore biosynthesis genes. Five microarrays comparing P. mirabilis HI4320 cultured in LB broth to P. mirabilis cultured in LB broth + 15 uM Desferal (an iron chelator) were analyzed. All five arrays are biological replicates; arrays #2 and 4 are dye swaps.
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