Project description:Regional variation in Swedish acid sulfate soil microbial communities is influenced by temperature, grain size, and organic matter content
| PRJEB63388 | ENA
Project description:EMG produced TPA metagenomics assembly of the Acid sulfate soil microbial profile - pre-investigation (Acid sulfate soil microbial profile - pre-investigation) data set.
Project description:Investigation of sulfur metabolism in Clostridium thermocellum DSM 1313 ∆hpt, to determine growth and gene expression when the organism is incubated with either the oxidized (i.e., sulfate) or the reduced and assimilated (i.e., cysteine) forms of sulfur. A sulfite reductase (∆hpt ∆SO3R) knockout mutant to limit sulfur assimilation was created to compare the resulting gene expression patterns by RNAseq transciptomics against the parental strain (∆hpt) when both are grown in the presence of sulfate. Additionally, we bypass the sulfate auxotrophy of the mutant by providing assimilated sulfur in the form of cysteine to determine whether growth is restored to normal and whether methionine can be biosynthesized by yet uncharacterized pathways in this organism.
Project description:The expression profile of natural isolate of S. cerevisiae carrying different copy of CUP2 were compared under cooper sulfate stress