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Single cell RNA-seq of human breast skin cells

ABSTRACT: Single cell RNA-seq of human breast skin cells

PROVIDER: PRJEB64504 | ENA |

REPOSITORIES: ENA

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Single cell RNA-seq of human breast skin cells

Project description:Cells were generated for a single cell atlas of adult human skin to dissect the cellular and molecular organisation underpinning human skin immune barrier function. Surplus skin from breast reconstruction surgery was collected (n=3), then the top 200 μm layer was taken. Epidermis was separated from the dermis, and both layers were separately digested. Single cells were FACS sorted into eight categories, then loaded onto a 10x Genomics Chromium Controller, and sequenced on an Illumina HiSeq 4000.

2021-02-10 | E-MTAB-8142 | biostudies-arrayexpress

Single-cell transcriptomics of human-skin-equivalent organoids

Project description:Single-cell transcriptomics of human-skin-equivalent organoids

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Single-cell RNA sequencing of emigrating cells from human psoriasis skin and control normal skin

Project description:Single-cell RNA sequencing is transforming how we understand skin immunology, but previous human skin single-cell RNA sequencing data included only a small fraction of inflammatory cells among the overall cell population, such that functional subsets may be difficult to ascertain. We have overcome these obstacles by harvesting inflammatory cells emigrating from a half of 6 mm punch biopsy skin after 48-hour incubation in culture medium without any enzyme, and then analyzing the harvested cells with single-cell RNA sequencing. By this strategy, we obtained single-cell RNA sequencing data of 24,354 cells (leukocytes 46.0%, keratinocytes 49.6%, and melanocytes 2.4%) from 13 human psoriasis skin and 5 healthy volunteer skin. Unsupervised clustering identified NK cells, CD161+ T-cells, CD8+ T-cells, CD4+ T-cells, regulatory T-cells, mature & semimature dendritic cells, melanocytes, and keratinocytes in different layers - S. (Stratum) corneum, S. granulosum, S. spinosum, and S. basale. To understand psoriasis immunopathogenesis at single-cell levels, we compared gene expression between psoriasis cells vs. control cells within each inflammatory cell subtype clusters.

2021-05-03 | GSE151177 | GEO

Single cell transcriptional zonation of human psoriasis whole skin

Project description:To explore the cellullar and molecular alteration of human psoriasis, we collected full-thickness skin from the lesion region of 3 patients and the similar region of 3 healthy donors, and submit for single cell RNA sequencing (scRNAseq) with 10x genomics (V3.1). The transcriptional landscape of human psoriasis whole skin provide a unique view of immuno-regulation among skin cell types. 1. "Single cell transcriptional zonation of human psoriasis skin identifies an alternative immunoregulatory axis",<Cell Death Dis.>, 2021 May 6;12(5):450. https://yz-studio.shinyapps.io/shinyapph5ad/ 2. "Integrative single-cell transcriptomic investigation unveils long non-coding RNAs associated with localized cellular inflammation in psoriasis" <Front Immunol>2023 Sep 26:14:1265517. Integrated dataset: 106,675 cells from 11 healthy human skin and 79,887 cells from 9 psoriatic human skin https://yz-studio.shinyapps.io/psoriaticskincellatlas2/

2021-04-26 | GSE162183 | GEO

Spatiotemporal Single-Cell Roadmap of Human Skin Wound Healing (Single-cell-Wound)

Project description:Spatiotemporal Single-Cell Roadmap of Human Skin Wound Healing (Single-cell-Wound)

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Spatiotemporal Single-Cell Roadmap of Human Skin Wound Healing (Single-cell-VU)

Project description:Spatiotemporal Single-Cell Roadmap of Human Skin Wound Healing (Single-cell-VU)

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Single cell transcriptional zonation of human psoriasis whole skin

Project description:Single cell transcriptional zonation of human psoriasis whole skin

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Single Cell RNA sequencing of Adult Human Breast Epithelial Cells

Project description:Single Cell RNA sequencing of Adult Human Breast Epithelial Cells

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Single-cell RNA sequencing of emigrating cells from human psoriasis skin and control normal skin

Project description:Single-cell RNA sequencing of emigrating cells from human psoriasis skin and control normal skin

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Human fetal liver, skin and kidney single cell transcriptome data

Project description:This is a droplet-based single cell transcriptome data set from 13 human fetal livers (6-18 PCW) and 8 skin and kidney samples (6-12 PCW). It includes 199,642 cells with a mean detected gene number of 3000.

2019-10-11 | E-MTAB-7407 | biostudies-arrayexpress

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