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Hi-C in the Drosophila wild-type embryos at 6-8 h and 16-18 hrs after egg-laying

ABSTRACT: Hi-C in the Drosophila wild-type embryos at 6-8 h and 16-18 hrs after egg-laying

PROVIDER: PRJEB64862 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR11807455_1.fastq.gz	Fastqsanger.gz
	ERR11807455_2.fastq.gz	Fastqsanger.gz
	ERR11807456_1.fastq.gz	Fastqsanger.gz
	ERR11807456_2.fastq.gz	Fastqsanger.gz
	ERR11807457_1.fastq.gz	Fastqsanger.gz

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Hi-C in the Drosophila wild-type embryos at 6-8 h and 16-18 hrs after egg-laying

Project description:Hi-C was performed on wild-type Drosophila melanogaster embryos at 6-8 hrs and 16-18 hrs after egg-laying, on 2 biological replicates per time point. Each library was first checked on MiSeq and then deeply sequenced on HiSeq 2000 (4 lanes for each biological replicate). This study completes the E-MTAB-12070 Hi-C dataset on 2-4 hrs wild-type Drosophila melanogaster embryos. Both studies use the same experimental procedures (protocols, sequencer model and run parameters)

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Hi-C in the Drosophila wild-type embryos at 2-4h after egg-laying

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Comparative transcriptome analysis reveals critical genes/pathways related yolk precursor synthesis in livers of Guangxi Ma chicken with different egg laying rate

Project description:Liver tissues of Guangxi Ma chicken from 32-week old with m performance, 50-week old with high and low laying performance, and 72-week old with high and low laying performance were collected and sequenced in quadruplicate using RNA-seq. The sequences were double-ended sequenced on the DNBSEQ sequencing platform. The sequence reads were quality controlled and then aligned with genomic sequences using HISAT2 program, quantified by featureCounts program, and gene expression levels were verified by qRT-PCR with SYBR Green detection. The results will be helpful to explore the factors that affecting laying performance from the perspective of yolk synthesis and provide a theoretical basis for improving the egg production of Guangxi Ma chicken.

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Life history shapes variation in egg composition in a wild passerine

Project description:Maternal investment directly shapes early developmental conditions and therefore has long-term fitness consequences for the offspring. In oviparous species prenatal maternal investment is fixed at the time of laying. To ensure the best survival chances for most of their offspring, females must equip their eggs with the resources required to perform well under various circumstances, yet the actual mechanisms remain unknown. Here we describe the blue tit egg albumen and yolk proteome and evaluate its potential to mediate maternal effects. We show that variation in egg composition (proteins, lipids, carotenoids) primarily depends on laying order, female age and paternity (within- versus extra-pair) and that the investment in the egg proteome is functionally biased among eggs. Our results suggest that maternal effects on egg composition result from both passive and active (partly compensatory) mechanisms, and that the between- and within-clutch variation in egg composition creates diverse biochemical environments for embryonic development.

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2022-03-19 | GSE198604 | GEO

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