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Transcription profiling of Drosophila Mef2 homozygous mutant embryos in a timecourse of embryogenesis

ABSTRACT: Expression profiling of Drosophila Mef2 homozygous mutant embryos in a timecourse of embryogenesis. Twelve one-hour timepoints were assayed (5-16 hrs, 18-19 hrs after egg laying). Homozygous mutant embryos were collected using GFP-activated embryo sorting at the same time as stage-matched wildtype controls. Four independent collections were performed at each timepoint. Total RNA was extracted and amplified. Mutant and respective wildtype samples were hybridized together on cDNA arrays.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Charles Girardot

PROVIDER: E-TABM-57 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A temporal map of transcription factor activity: mef2 directly regulates target genes at all stages of muscle development.

Sandmann Thomas T Jensen Lars J LJ Jakobsen Janus S JS Karzynski Michal M MM Eichenlaub Michael P MP Bork Peer P Furlong Eileen E M EE

Developmental cell 20060601 6

Dissecting components of key transcriptional networks is essential for understanding complex developmental processes and phenotypes. Genetic studies have highlighted the role of members of the Mef2 family of transcription factors as essential regulators in myogenesis from flies to man. To understand how these transcription factors control diverse processes in muscle development, we have combined chromatin immunoprecipitation analysis with gene expression profiling to obtain a temporal map of Mef ...[more]

PMID: 16740481

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