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Whole Exome Sequencing Data of Dabrafenib Resistant HT-29 Cell Line

ABSTRACT: Whole Exome Sequencing Data of Dabrafenib Resistant HT-29 Cell Line

PROVIDER: PRJEB65911 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR12026946_1.fastq.gz	Fastqsanger.gz
	ERR12026946_2.fastq.gz	Fastqsanger.gz
	ERR12026947_1.fastq.gz	Fastqsanger.gz
	ERR12026947_2.fastq.gz	Fastqsanger.gz

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Whole Exome Sequencing Data of Dabrafenib Resistant HT-29 Cell Line

Project description:HT-29 cells were barcoded using the CloneTracker lentiviral barcode library and then dabrafenib resistant derivatives of these cell lines were established, respectively. Five million barcoded HT-29 cells were seeded into 15 cm cell culture dishes. When the cells reached confluency, two million cells per dish were seeded into four different 15 cm dishes (DMSO Control, Replica A, B, C) and two million cell pellets were stocked as initial cell population. Harvesting used medium through the experiment was performed at monthly intervals. Barcoded HT-29 cell line replicates A, B, and C were treated with 2XIC50 (199.6 nM) of dabrafenib concentration for the duration of 3 months.Barcoded data can be accessed via accession code E-MTAB-13018. Whole exome sequencing of dabrafenib-resistant A replicate and DMSO control cell lines were carried out.

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