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Small RNAseq - Plant parasitic cyst nematodes respond to viral infection through siRNA activation

ABSTRACT: small RNAseq - Plant parasitic cyst nematodes respond to viral infection through siRNA activation

PROVIDER: PRJEB66168 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR12050910.fastq.gz	Fastqsanger.gz
	ERR12050911.fastq.gz	Fastqsanger.gz
	ERR12050912.fastq.gz	Fastqsanger.gz

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small RNAseq - Plant parasitic cyst nematodes respond to viral infection through siRNA activation

Project description:In this project we sequenced smallRNAs to see how both BCN (H.schachtii) and PCN (G. rostochiensis line 19 and 22) react to different viruses. After adapter removal, small RNA analysis was performed on sequences from 18 to 28 nt in length. Also the smallRNAs are mapped to the reference genomes of the nematodes to look for smallRNAs.

2023-12-25 | E-MTAB-13351 | biostudies-arrayexpress

Plant parasitic nematodes single embryo RNA sequencing

Project description:In order to study the similarities and differences in embryonic development between plant-parasitic nematodes and free-living nematodes, we performed RNA-seq on embryos of three plant-parasitic nematodes at a total of 11 stages from the single-cell stage to the J1 stage

2024-05-25 | GSE268038 | GEO

Microarrays of Female and Male cyst nematode infected Arabidopsis root samples

Project description:Plant-parasitic cyst nematodes induce syncytial cells in the roots of their host plants. Cyst nematodes are sexually dimorphic, with their differentiation into male or femaleis strongly influenced by host environmental conditions. Under favorable conditions with plenty of nutrients, more females develop, whereas mainly male nematodes develop under adverse conditions, such as in resistant plants. We collected root segments containing male-associated syncytia (MAS) or female-associated syncytia (FAS), isolated syncytial cells by laser microdissection, and performed a comparative transcriptome analysis using Microarrays.

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DNA hypomethylation confers enhanced immunity against parasitic nematodes in plants

Project description:Background: Epigenetic processes play an important role in the plant response to adverse environmental conditions. A role for DNA hypomethylation has recently been suggested in the pathogenic interaction between bacteria and plants, yet it remains unclear whether this phenomenon reflects a conserved and general plant immunity response. We therefore investigated the role of DNA methylation in the plant defence against damaging parasitic nematodes. Methods and results: Treatment of roots of rice (monocot plant) and tomato (dicot plant) by a nematode-associated molecular pattern (NAMP) from different parasitic nematodes revealed global DNA hypomethylation using ELISA based quantification, suggesting conservation among plants. Focusing on root-knot induced gall tissue in rice, the causal impact of hypomethylation on immunity was revealed by a significantly reduced plant susceptibility upon 5-Azacitidine treatment. Whole genome bisulfite sequencing revealed that hypomethylation was massively present in the CHH context, while absent for CpG or CHG nucleotide contexts. CHH hypomethylated regions were predominantly associated with gene promoter regions, which was not correlated with activated gene expression at the same time point, but rather showed a delayed effect on transcriptional gene activation. Finally, the relevance of CHH hypomethylation in plant defence was confirmed in rice mutants of the RNA-directed DNA methylation pathway (RdDM) and DDM1, which are known to be steering DNA methylation in CHH context. Conclusions: We demonstrated that DNA hypomethylation confers enhanced defence in rice towards root-parasitic nematodes and is likely to be part of the basal NAMP-triggered immunity response in plants.

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Plant Parasitic Nematodes

Project description:Plant parasitic nematode genome sequencing

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Gene expression in populations of the potato cyst nematode Globodera pallida in diapause or quiescence following hydration or exposure to tomato root diffusate

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An extracellular Argonaute protein mediates export of repeat-associated small RNAs into vesicles in parasitic nematodes

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2019-02-21 | GSE117169 | GEO

Transcriptome analysis of Heterodera avenae two developmental stages

Project description:The cereal cyst nematode (CCN, Heterodera avenae) is a major pest of wheat (Triticum spp) that reduces crop yields in many countries. Cyst nematodes are obligate sedentary endoparasites that reproduce by amphimixis. Here, we report the first transcriptome analysis of two parasitic stages of H. avenae.

2014-01-27 | E-MTAB-2221 | biostudies-arrayexpress

DNA hypomethylation confers enhanced immunity against parasitic nematodes in plants

Project description:DNA hypomethylation confers enhanced immunity against parasitic nematodes in plants

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