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Role of phage ϕ1 in two strains of Salmonella Rissen, sensitive and resistant to phage ϕ1

ABSTRACT: Role of phage ϕ1 in two strains of Salmonella Rissen, sensitive and resistant to phage ϕ1

PROVIDER: PRJEB69481 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	ERR12268042_1.fastq.gz	Fastqsanger.gz
	ERR12268042_2.fastq.gz	Fastqsanger.gz
	ERR12268043_1.fastq.gz	Fastqsanger.gz
	ERR12268043_2.fastq.gz	Fastqsanger.gz

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Role of phage ϕ1 in two strains of Salmonella Rissen, sensitive and resistant to phage ϕ1.

Project description:BackgroundThe study describes the Salmonella Rissen phage ϕ1 isolated from the ϕ1-sensitive Salmonella Rissen strain RW. The same phage was then used to select the resistant strain RRϕ1+, which can harbour or not ϕ1.ResultsFollowing this approach, we found that ϕ1, upon excision from RW cells with mitomycin, behaves as a temperate phage: lyses host cells and generates phage particles; instead, upon spontaneous excision from RRϕ1+ cells, it does not generate phage particles; causes loss of phage resistance; switches the O-antigen from the smooth to the rough phenotype, and favors the transition of Salmonella Rissen from the planktonic to the biofilm growth. The RW and RRϕ1+ strains differ by 10 genes; of these, only two (phosphomannomutase_1 and phosphomannomutase_2; both involved in the mannose synthesis pathway) display significant differences at the expression levels. This result suggests that phage resistance is associated with these two genes.ConclusionsPhage ϕ1 displays the unusual property of behaving as template as well as lytic phage. This feature was used by the phage to modulate several phases of Salmonella Rissen lifestyle.

| S-EPMC6286511 | biostudies-literature

Salmonella Rissen

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Salmonella enterica subsp. enterica serovar Rissen isolate Salmonella Rissen

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Comparative genomic hybridization of twenty four Salmonella enteritidis strains from six phage types

Project description:In this study differences at the genetic level of 24 S. Enteritidis strains from six phage types were compared using Comparative Genomic Hybridisation (CGH) DNA microarrays to determine why some certain phage types are 'less successful' in causing human infection.

2008-08-02 | E-MEXP-1720 | biostudies-arrayexpress

Genomic and gene-expression comparisons among phage-resistant type-IV pilus mutants of Pseudomonas syringae pathovar phaseolicola

Project description:Pseudomonas syringae pv. phaseolicola (Pph) is a significant bacterial pathogen of agricultural crops, and phage Î¦6 and other members of the dsRNA virus family Cystoviridae undergo lytic (virulent) infection of Pph, using the type IV pilus as the initial site of cellular attachment. Despite the popularity of Pph/phage Î¦6 as a model system in evolutionary biology, Pph resistance to phage Î¦6 remains poorly characterized. To investigate differences between phage Î¦6 resistant Pseudomonas syringae pathovar phaseolicola strains, we performed expression analysis of super and non piliated strains of Pseudomonas syringae to determine the genetic cause of resistance to viral infection.

2016-03-15 | E-MTAB-4053 | biostudies-arrayexpress

Phage resistant genomes

Project description:Pathogen genomic surveillance as a scalable framework for precision phage therapy - phage resistant genomes

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Response to low-level phage predation in Staphylococcus aureus biofilms

Project description:An important lesson from the war on pathogenic bacteria has been the need to understand the physiological responses and evolution of natural microbial communities. Bacterial populations in the environment are generally forming biofilms subject to some level of phage predation. These multicellular communities are notoriously resistant to antimicrobials and, consequently, very difficult to eradicate. This has sparked the search for new therapeutic alternatives, including phage therapy. This study demonstrates that S. aureus biofilms formed in the presence of a non-lethal dose of phage phiIPLA-RODI exhibit a unique physiological state that could potentially benefit both the host and the predator. Thus, biofilms formed under phage pressure are thicker and have a greater DNA content. Also, the virus-infected biofilm displayed major transcriptional differences compared to an untreated control. Significantly, RNA-seq data revealed activation of the stringent response, which could slow down the advance of the bacteriophage within the biofilm. The end result would be an equilibrium that would help bacterial cells to withstand environmental challenges, while maintaining a reservoir of sensitive bacterial cells available to the phage upon reactivation of the dormant carrier population.

2017-01-20 | GSE87706 | GEO

Salmonella enterica subsp. enterica serovar Rissen strain:B1905 | isolate:B1905

Project description:Whole genome sequencing of Salmonella enterica subsp. enterica serovar Rissen(Salmonella Rissen)

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Analysis of the genomic composition of phage YerA41

Project description:Genomic material isolated from purified phage YerA41 lysate was shown to contain RNA. YerA41 phage lysate was RNase treated to remove phage-external RNA and total RNA was then isolated from the phage preparate using Qiagen Rneasy mini kit. The isolated RNA was sequenced to elucidate its origin. The results suggested that the RNA originated from intact ribosomes of the host bacterium that contaminated the phage lysate.

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The transcriptional program of Staphylococcus aureus phage K is affected by a host rpoC mutation that confers phage K resistance

Project description:To better understand host/phage interactions and the genetic bases of phage resistance in a model system relevant to potential phage therapy, we isolated several spontaneous mutants of the USA300 S. aureus clinical isolate NRS384 that were resistant to phage K. Six of these had a single missense mutation in the host rpoC gene, which encodes the RNA polymerase beta prime subunit. To examine the hypothesis that the mutations in the host RNA polymerase affect the transcription of phage genes, we performed RNA-seq analysis on total RNA samples collected from NRS384 wild-type (WT) and rpoC G17D mutant cultures infected with phage K, at different time points after infection. Infection of the WT host led to a steady increase of phage transcription relative to the host. Our analysis allowed us to define different early, middle, and late phage genes based on their temporal expression patterns and group them into transcriptional units. Predicted promoter sequences defined by conserved -35, -10, and in some cases extended -10 elements were found upstream of early and middle genes. However, sequences upstream of late genes did not contain clear, complete, canonical promoter sequences, suggesting that factors in addition to host RNA polymerase are required for their regulated expression. Infection of the rpoC G17D mutant host led to a transcriptional pattern that was similar to the WT at early time points. However, beginning at 20 minutes after infection, transcription of late genes (such as phage structural genes and host lysis genes) was severely reduced. Our data indicate that the rpoCG17D mutation prevents the expression of phage late genes, resulting in a failed infection cycle for phage K. In addition to illuminating the global transcriptional landscape of phage K throughout the infection cycle, these studies can inform our investigations into the bases of phage K’s control of its transcriptional program as well as mechanisms of phage resistance.

2024-10-09 | GSE253516 | GEO

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