HLA and KIR haplotype sequencing
Ontology highlight
ABSTRACT:
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| ERR588050_1.fastq.gz | Fastqsanger.gz | |||
| ERR588050_2.fastq.gz | Fastqsanger.gz | |||
| ERR588051_1.fastq.gz | Fastqsanger.gz | |||
| ERR588051_2.fastq.gz | Fastqsanger.gz | |||
| ERR588052_1.fastq.gz | Fastqsanger.gz |
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Project description:Rapid advances in biochemical technologies have enabled several strategies for typing candidate HLA alleles, but linking them into a single MHC haplotype structure remains challenging. Here we have developed a multi-loci haplotype phasing technique and demonstrate its utility towards phasing of MHC and KIR loci in human samples. We accurately (~99%) reconstruct the complete haplotypes for over 90% of sequence variants spanning the 4-megabase region of these two loci. By haplotyping a majority of coding and non-coding alleles at the MHC and KIR loci in a single assay, this method has the potential to assist transplantation matching and facilitate investigation of the genetic basis of human immunity and disease. Complete haplotype phasing of 2 loci (MHC and KIR) in 1 human cell line.
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