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Complete Haplotype Phasing of the MHC and KIR Loci with Targeted HaploSeq

ABSTRACT: Rapid advances in biochemical technologies have enabled several strategies for typing candidate HLA alleles, but linking them into a single MHC haplotype structure remains challenging. Here we have developed a multi-loci haplotype phasing technique and demonstrate its utility towards phasing of MHC and KIR loci in human samples. We accurately (~99%) reconstruct the complete haplotypes for over 90% of sequence variants spanning the 4-megabase region of these two loci. By haplotyping a majority of coding and non-coding alleles at the MHC and KIR loci in a single assay, this method has the potential to assist transplantation matching and facilitate investigation of the genetic basis of human immunity and disease.

ORGANISM(S): Homo sapiens

PROVIDER: GSE65726 | GEO | 2016/02/01

SECONDARY ACCESSION(S): PRJNA274802

REPOSITORIES: GEO

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Complete Haplotype Phasing of the MHC and KIR Loci with Targeted HaploSeq

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