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Sponge development

ABSTRACT: Sponge development

PROVIDER: PRJEB7138 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	ERR592859_1.fastq.gz	Fastqsanger.gz
	ERR592859_2.fastq.gz	Fastqsanger.gz
	ERR592860_1.fastq.gz	Fastqsanger.gz
	ERR592860_2.fastq.gz	Fastqsanger.gz
	ERR592861_1.fastq.gz	Fastqsanger.gz

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sponge development

Project description:sponge development

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Project description:In order to compare sponge and eumetazoan (higher animal) body plans, we identified and studied expression of a broad range of eumetazoan developmental regulatory genes in Sycon ciliatum (Calcispongiae). In this species, embryonic development is semi-synchronous within a population, synchronous within individuals, and oocytes and embryos occupy a significant fraction of the volume of the sponges during the reproductive period. RNASeq libraries representing non-reproductive (somatic) tissue slices along the body axis, as well as oocytes, embryos and free swimming larvae were generated from material obtained by sampling throughout the life cycle.

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Regeneration in sponge Sycon ciliatum partly mimics postlarval development

Project description:Regeneration in sponge Sycon ciliatum partly mimics postlarval development

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Expression data of lentivirus sponge Protein Arginine Methyltransferase 5 downregulated NCI-H929 cell line compared with control lentivirus sponge

Project description:Multiple myeloma is characterized by frequent chromosomal alterations. Protein Arginine Methyltransferase 5 (PRMT5) catalyzes symmetric dimethylation of arginine, a post-translational modification involved in cancer and embryonic development. We sought to functionally validate the role of PRMT5 and delineate their downstream target genes in MM. Using “sponge” lentiviral vectors to knock down PRMT5 in vitro and in vivo, we have documented enhanced apoptosis and descend proliferation of MM cells compared with control lentivirus sponge . Expression profiling analysis of PRMT5-deficient cells identified a large number of downstream target genes, which provides a valuable tool to investigate their function in MM pathogenesis and their potential use as therapeutic targets.

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