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RNA-seq of Campylobacter jejuni 11168 wild-type and Cj1608 deletion mutant under microaerophilic and paraquat stress conditions

ABSTRACT: RNA-seq of Campylobacter jejuni 11168 wild-type and Cj1608 deletion mutant under microaerophilic and paraquat stress conditions

PROVIDER: PRJEB71435 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR12380079_1.fastq.gz	Fastqsanger.gz
	ERR12380079_2.fastq.gz	Fastqsanger.gz
	ERR12380080_1.fastq.gz	Fastqsanger.gz
	ERR12380080_2.fastq.gz	Fastqsanger.gz
	ERR12380081_1.fastq.gz	Fastqsanger.gz

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RNA-seq of Campylobacter jejuni 11168 wild-type and Cj1608 deletion mutant under microaerophilic and paraquat stress conditions

Project description:We performed transcriptome (RNA-seq) analyses for Campylobacter jejuni 11168 wild-type (WT) and Cj1608 deletion mutant (ΔCj1608::aphA-3) under oxidative stress and optimal microaerobic growth conditions. The expression of 231 genes was affected by oxidative stress in stressed wild-type cells (WTS) compared to non-stressed cells (WT). A comparison of genes expressed in the ΔCj1608 and WT strains under optimal growth conditions revealed 380 differently expressed genes. Moreover, transcriptional changes and overall final protein levels correlated across multiple genes. The data were validated through RT-qPCR and phenotype experiments for selected processes.

2024-06-14 | E-MTAB-13650 | biostudies-arrayexpress

Campylobacter jejuni comparative genome: control strain C. jejuni subsp. jejuni ATCC 700819 (NCTC 11168) vs. 12 other strains

Project description:Gene content comparison of control C. jejuni subsp. jejuni strain 11168 which colonizes and causes disease in C57BL/6 IL-10-/- mice versus C. jejuni strains D6844, D6845, D6846, D6847, D6848, D6849, D0121, D0835, D2586, D2600,33560 and NW in the C57BL/6 IL-10-/- mice. Keywords: DNA/DNA comparison

2010-10-21 | GSE24764 | GEO

Differential RNA-sequencing of Campylobacter jejuni NCTC 11168

Project description:We report the use of differential RNA-sequencing for the determination of the primary transcriptome of wildtype Campylobacter jejuni NCTC 11168. This allows for the genome-wide determination of transcription start sites.

2013-09-13 | GSE49312 | GEO

RNA-sequencing of Campylobacter jejuni NCTC 11168 wildtype and fur perR mutant

2014-07-01 | GSE49687 | GEO

Addition of Exogenous AI-2 to C. jejuni 11168 Wild Type and LuxS01.

Project description:Transcriptional profiling of Campylobacter jejuni NCTC 11168 wild type and LuxS01 mutant strains comparing the effects of exogenously added in vitro-produced autoinducer 2 (AI-2) versus a control buffer to both strains.

2009-10-08 | GSE18427 | GEO

Campylobacter jejuni exposure to 0.25mM GSNO

Project description:Batch cultures of Wild-type C. jejuni NCTC 11168 were grown in 150 ml volumes of Mueller-Hinton broth in 250 ml baffled flasks. Microaerophilic conditions were generated using a MACS-VA500 microaerophilic work station (10 % Oxygen, 10 % Carbon dioxide, 80 % Nitrogen) from Don Whitley Scientific, Ltd which also maintained the growth temperature at 42 ºC. When mid-exponential phase was reached 0.25 mM GSNO was added to half of the cultures. After a 5/10/15/45 minute exposure samples of both treated and untreated cells were harvested into phenol/ethanol to stabilize the RNA and total RNA was purified using Qiagen’s RNeasy Mini kit (as recommended by the suppliers) prior to use in microarray analysis. Keywords: Time Course

2007-02-17 | GSE7048 | GEO

Screening for novel immunogenic proteins of Campylobacter jejuni NCTC 11168

Project description:The screening of a cDNA derived expression library of Campylobacter jejuni NCTC 11168 expressed in E. coli using a fusion construct and specific HaloTag interaction to a modified surface is shown. 1536 different clones were screened including positive (hisJ, cjaA, peb1a) and negative (argC, pyrC, gapA) reference proteins. The goal of the screening was to identify potential novel immunogenic proteins from C. jejuni by selecting clones showing a high signal intensity in comparison to the known antigens used as positive markers. Afterwards, the most promising clones were sequenced to identify the gene and corresponding protein, and these proteins were then investigated further. Consequently, 22 novel immunogenic proteins could be identified.

2013-04-06 | GSE45268 | GEO

RNA-sequencing of Campylobacter jejuni NCTC 11168 wildtype and fur perR mutant

Project description:We report the use of RNA-seq analysis for the determination of RPKM transcript levels in wildtype and fur perR mutant of Campylobacter jejuni NCTC 11168. This allows for comparison of gene expression levels. Campylobacter jejuni NCTC 11168 wildtype and fur perR mutant were grown to late log phase, RNA was purified and used for RNA-sequencing by Illumina HiSeq sequencing

2014-07-01 | E-GEOD-49687 | biostudies-arrayexpress

Comparison of the genome-sequenced variant of Campylobacter jejuni NCTC 11168 and the original clonal clinical isolate

Project description:Abstract: The genome sequence of the enteric bacterial pathogen Campylobacter jejuni NCTC 11168 (11168-GS) was published in 2000, providing a valuable resource for the identification of C. jejuni-specific colonization and virulence factors. Surprisingly, the 11168-GS clone was subsequently found to colonize 1-day-old chicks following oral challenge very poorly compared to other strains. In contrast, we have found that the original clinical isolate from which 11168-GS was derived, 11168-O, is an excellent colonizer of chicks. Other marked phenotypic differences were also identified: 11168-O invaded and translocated through tissue culture cells far more efficiently and rapidly than 11168-GS, was significantly more motile, and displayed a different morphology. Serotyping, multiple high-resolution molecular genotyping procedures, and subtractive hybridization did not yield observable genetic differences between the variants, suggesting that they are clonal. However, microarray transcriptional profiling of these strains under microaerobic and severely oxygen-limited conditions revealed dramatic expression differences for several gene families. Many of the differences were in respiration and metabolism genes and operons, suggesting that adaptation to different oxygen tensions may influence colonization potential. This correlates biologically with our observation that anaerobically priming 11168-GS or aerobically passaging 11168-O caused an increase or decrease, respectively, in colonization compared to the parent strain. Expression differences were also observed for several flagellar genes and other less well-characterized genes that may participate in motility. Targeted sequencing of the sigma factors revealed specific DNA differences undetected by the other genomic methods This SuperSeries is composed of the SubSeries listed below.

2005-12-31 | GSE4387 | GEO

Campylobacter jejuni subsp. jejuni NCTC 11168-PO

Project description:Campylobacter jejuni subsp. jejuni NCTC 11168-PO Genome sequencing

| PRJNA159829 | ENA