Project description:BackgroundApple production in Sweden and elsewhere is being threatened by the fungus, Neonectria ditissima, which causes a disease known as European canker. The disease can cause extensive damage and the removal of diseased wood and heavily infected trees can be laborious and expensive. Currently, there is no way to eradicate the fungus from infected trees and our knowledge of the infection process is limited. Thus, to target and modify genes efficiently, the genetic transformation technique developed for N. ditissima back in 2003 was modified.ResultsThe original protocol from 2003 was upgraded to use enzymes currently available in the market for making protoplasts. The protoplasts were viable, able to uptake foreign DNA, and able to regenerate back into a mycelial colony, either as targeted gene-disruption mutants or as ectopic mutants expressing the green fluorescent protein (GFP).ConclusionsA new genetic transformation protocol has been established and the inclusion of hydroxyurea in the buffer during the protoplast-generation step greatly increased the creation of knockout mutants via homologous recombination. Pathogenicity assays using the GFP-mutants showed that the mutants were able to infect the host and cause disease.

| S-EPMC9373326 | biostudies-literature

Draft Genome Sequence of a European Isolate of the Apple Canker Pathogen Neonectria ditissima.

Project description:The Sordariomycetes fungus Neonectria ditissima is a major pathogen of apples, causing canker on trees and fruit spoilage. We report here the draft genome sequence of a European strain isolated from cankerous tissue.

| S-EPMC4653771 | biostudies-literature

Fungal and bacterial endophytes in apple trees

Project description:This study shows that the diversity of fungal and bacterial endophytes in apple trees is influenced by site and tree genotype and associated with susceptibility to Neonectria ditissima

| PRJEB44670 | ENA

Neonectria

Project description:Neonectria sp. overview

| PRJNA1202250 | ENA

Neonectria lugdunensis

Project description:Neonectria lugdunensis overview

| PRJNA1149140 | ENA

The nature of resistance to Neonectria ditissima in apple

Project description:An investigation into genetic variants associated with improved tolerance to the fungal pathogen Neoectria ditissima in apple

2022-07-15 | PRJEB54689 | EVA