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Neonectria ditissima

ABSTRACT: Neonectria ditissima overview

PROVIDER: PRJNA298265 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
		Other
	SRR2154279_1.fastq.gz	Fastqsanger.gz
	SRR2154279_2.fastq.gz	Fastqsanger.gz
	SRR5227890_1.fastq.gz	Fastqsanger.gz
	SRR5227890_2.fastq.gz	Fastqsanger.gz

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Transformation and gene-disruption in the apple-pathogen, Neonectria ditissima.

Project description:BackgroundApple production in Sweden and elsewhere is being threatened by the fungus, Neonectria ditissima, which causes a disease known as European canker. The disease can cause extensive damage and the removal of diseased wood and heavily infected trees can be laborious and expensive. Currently, there is no way to eradicate the fungus from infected trees and our knowledge of the infection process is limited. Thus, to target and modify genes efficiently, the genetic transformation technique developed for N. ditissima back in 2003 was modified.ResultsThe original protocol from 2003 was upgraded to use enzymes currently available in the market for making protoplasts. The protoplasts were viable, able to uptake foreign DNA, and able to regenerate back into a mycelial colony, either as targeted gene-disruption mutants or as ectopic mutants expressing the green fluorescent protein (GFP).ConclusionsA new genetic transformation protocol has been established and the inclusion of hydroxyurea in the buffer during the protoplast-generation step greatly increased the creation of knockout mutants via homologous recombination. Pathogenicity assays using the GFP-mutants showed that the mutants were able to infect the host and cause disease.

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Draft Genome Sequence of a European Isolate of the Apple Canker Pathogen Neonectria ditissima.

Project description:The Sordariomycetes fungus Neonectria ditissima is a major pathogen of apples, causing canker on trees and fruit spoilage. We report here the draft genome sequence of a European strain isolated from cankerous tissue.

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Neonectria sp. DH2

Project description:Neonectria sp. DH2 Genome sequencing and assembly

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Draft Genome Sequences of Two Isolates of the Plant-Pathogenic Fungus Neonectria ditissima That Differ in Virulence.

Project description:Neonectria ditissima is the causal agent of apple canker. Here, we present the draft genome sequences of two isolates of N. ditissima that differ in virulence. Comparative genomics will enable pathogenicity determinants to be identified in this plant-pathogenic fungus.

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