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PyBrePsyg

ABSTRACT: Breviolum psygmophilum

PROVIDER: PRJEB72594 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
	ERR12654649_1.fastq.gz	Fastqsanger.gz
	ERR12654649_2.fastq.gz	Fastqsanger.gz
	ERR12654650_1.fastq.gz	Fastqsanger.gz
	ERR12654650_2.fastq.gz	Fastqsanger.gz
	ERR12666666.fastq.gz	Fastqsanger.gz

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Project description:Samples are from a screening experiment on 5 laboratory cultures of the coral endosymbiont: Symbiodiniaceae (Symbiodinium linuchae, Breviolum psygmophilum, Durusdinium trenchii, Effrenium voratum and Fugacium kawagutii). Samples are also from a thermal stress experiment (increased temperatures from 26 to 32 degrees C) carried out on Durusdinium trenchii and Cladocopium goreaui, two common coral endosymbionts on the Great Barrier Reef. All samples were collected on Markes Tenax TA thermal desorption tubes. Lawson, C.A., Possell, M., Seymour, J.R., Raina, J.B. and Suggett, D.J., 2019. Coral endosymbionts (Symbiodiniaceae) emit species-specific volatilomes that shift when exposed to thermal stress. Scientific reports, 9(1), pp.1-11.

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Project description:Dinoflagellate chromosomes represent a unique evolutionary experiment, as they exist in a permanently condensed, liquid crystalline state, are not packaged by histones, and contain genes organized into polycistronic arrays, with minimal transcriptional regulation. We analyze the 3D genome of {Breviolum minutum}, and find large topological domains without chromatin loops, demarcated by convergent gene array boundaries (``dinoTADs’’). Transcriptional inhibition degrades dinoTADs, implicating transcription-induced supercoiling as the primary topological force in dinoflagellates.

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