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UoEpiScra

ABSTRACT: Epithemia sp. CRS-2021a (pennate diatom), genomic and transcriptomic data

PROVIDER: PRJEB72603 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	ERR12654652_1.fastq.gz	Fastqsanger.gz
	ERR12654652_2.fastq.gz	Fastqsanger.gz
	ERR12654653_1.fastq.gz	Fastqsanger.gz
	ERR12654653_2.fastq.gz	Fastqsanger.gz
	ERR12653961_1.fastq.gz	Fastqsanger.gz

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uoEpiScra

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uoEpiScrs

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uoEpiScra1.metagenome

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Project description:Epithemia sp. CRS-2021b (pennate diatom)

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uoEpiScrs1

Project description:Epithemia sp. CRS-2021b (pennate diatom) genome assembly, uoEpiScrs1

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Investigating mixotrophic metabolism in the model diatom Phaeodactylum tricornutum.

Project description:Diatoms are prominent marine microalgae, interesting not only from an ecological point of view, but also for their possible use for biotechnology applications. They can be cultivated in phototrophic conditions, using sunlight as the only energy source. Some diatoms, however, can also grow in mixotrophic mode, where both light and external reduced carbon contribute to biomass accumulation. In this study, we investigated the consequences of mixotrophy on the growth and metabolism of the pennate diatom Phaeodactylum tricornutum, using glycerol as a source of reduced carbon. Transcriptomic, metabolomic and physiological data indicate that glycerol affects the central-carbon, carbon-storage and lipid metabolism of the diatom. In particular, glycerol addition mimics some typical responses of nitrogen limitation on lipid metabolism at the level of TAG accumulation and fatty acid composition. However, this compound does not diminish photosynthetic activity and cell growth, at variance with nutrient limitation, revealing essential aspects of the metabolic flexibility of these microalgae and suggesting possible biotechnological applications of mixotrophy.

2017-03-31 | GSE91004 | GEO

Early nutrient starvation response of the diatom Phaeodactylum tricornutum

Project description:An experiment was performed to determine the similarities on the RNA level between different conditions where cell division stops in the diatom Phaeodactylum tricornutum. Many of these conditions also increase the accumulation of lipids within the cell or impair photosynthesis. The different metabolic responses were evaluated and the dataset was mined for potential transcriptional regulators of these changes. The experimental setup was as follows: Cells from the pennate diatom Phaeodactylum tricornutum were grown in ESAW medium under continous fluorescent light at 21C in baffled shakeflasks. Exponentially growing cells were harvested by centrifugation and washed twice in 21gr/L NaCL to remove nutrients. Cells were subsequently resuspended in the five different media/conditions (control, darkness, no nitrate, no phosphate, nocodazole).

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