Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Bacterial 16S rRNA community in manganese reducing slurry incubation experiment with sediment of Potter Cove

ABSTRACT: Bacterial 16S rRNA community in manganese reducing slurry incubation experiment with sediment of Potter Cove

PROVIDER: PRJEB72882 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR12687400_1.fastq.gz	Fastqsanger.gz
	ERR12687400_2.fastq.gz	Fastqsanger.gz
	ERR12687401_1.fastq.gz	Fastqsanger.gz
	ERR12687401_2.fastq.gz	Fastqsanger.gz
	ERR12687402_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 504

Similar Datasets

Bacterial 16S rRNA community in sulfate-reducing stable isotope probing slurry incubation experiment testing different electron donors with sediment of Potter Cove

Project description:Bacteria active during sulfate-reducing incubations with Potter Cove sediment

| PRJEB83594 | ENA

Bacterial 16S rRNA community in acetate degrading stable isotope probing slurry incubation experiment across temperature range with sediment of Potter Cove

Project description:Acetate degrading bacteria across temperature in Potter Cove sediment

| PRJEB82428 | ENA

2020 Potter Cove, Antarctica metagenomes

Project description:Potter Cove (King George Island, Antarctic Peninsula) metagenomic dataset from 0-60m depth sampled on January and February 2020

| PRJEB61010 | ENA

Expanding the Pathways of Manganese Homeostasis: Role of a Small Manganese Chaperone Protein, MntS

Project description:Escherichia coli possesses >65 small proteins of <50 amino acids, many of which are uncharacterized. We have identified a new small protein, MntS, involved in manganese homeostasis. Manganese is a critical micronutrient, serving as an enzyme cofactor and protecting against oxidative stress. Yet manganese is toxic in excess and little is known about its function in cells. Bacteria carefully control intracellular manganese levels using the transcription regulator MntR. Before this work, mntH, which encodes a manganese importer, was the only gene known to respond to manganese via MntR repression in E. coli K12. We demonstrated that mntS is another member of the MntR manganese regulon. We also identified yebN, which encodes a putative manganese efflux pump, as the first gene positively regulated by MntR in Enterobacteria. Since MntS is expressed when manganese levels are low, causes manganese sensitivity when overexpressed, and binds manganese, we propose that MntS may be a manganese chaperone. This study reveals new factors involved in manganese regulation and metabolism and expands our knowledge of how small proteins function. Two E. coli strains, MG1655 (wild type) and GSO458 (Delta-mntR) were grown to OD600 ~ 0.5 in M9 glucose media at 37 M-BM-:C and treated with 10 microM MnCl2. In the first experiment, this incubation with 10 microM MnCl2 was for 60 min and in the second experiment, it was for 10 min. RNA was extracted using the hot phenol method and cDNA prepared and hybridized according the manufacturer's instructions (Affymetrix).

2011-12-08 | E-GEOD-25318 | biostudies-arrayexpress

Bacterial 16S rRNA sequencing sediment samples core STA14.04, Potter Cove, King George Island/Isla 25 de Mayo

Project description:Bacterial 16S rRNA sequencing sediment samples core STA14.04, Potter Cove, King George Island/Isla 25 de Mayo

| PRJEB83592 | ENA

Bacterial 16S rRNA gene sequencing sediment samples at STA14, Potter Cove, King George Island/Isla 25 de Mayo

Project description:Bacterial 16S rRNA gene sequencing sediment samples at STA14, Potter Cove, King George Island/Isla 25 de Mayo

| PRJEB83593 | ENA

Bacterial 16S rRNA gene sequencing sediment samples core STA01.02 Potter Cove, King George Island/Isla 25 de Mayo

Project description:Bacterial 16S rRNA gene sequencing sediment samples core STA01.02 Potter Cove, King George Island/Isla 25 de Mayo

| PRJEB72873 | ENA

B. japonicum USDA110 low manganese vs. high manganese

Project description:Transcriptional profiling of B. japonicum manganese responsive cells and mur mutant responsive cells 3 independent biological materials were prepared for Wt manganese deplete, replete cultured cells and mur mutant manganese replete cultured cells

2011-08-24 | E-GEOD-30932 | biostudies-arrayexpress

Expanding the Pathways of Manganese Homeostasis: Role of a Small Manganese Chaperone Protein, MntS

2011-12-08 | GSE25318 | GEO

B. japonicum USDA110 low manganese vs. high manganese

Project description:Transcriptional profiling of B. japonicum manganese responsive cells and mur mutant responsive cells

2011-08-25 | GSE30932 | GEO