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MRNA-Seq of Danio rerio exposed to different concentrations of Fulvestrant against untreated control groups

ABSTRACT: mRNA-Seq of Danio rerio exposed to different concentrations of Fulvestrant against untreated control groups

PROVIDER: PRJEB75017 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	ERR12916371_1.fastq.gz	Fastqsanger.gz
	ERR12916371_2.fastq.gz	Fastqsanger.gz
	ERR12916372_1.fastq.gz	Fastqsanger.gz
	ERR12916372_2.fastq.gz	Fastqsanger.gz
	ERR12916373_1.fastq.gz	Fastqsanger.gz

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mRNA-Seq of Danio rerio exposed to different concentrations of Fulvestrant against untreated control groups

Project description:In the present study transcriptome analysis was employed to investigate the early molecular responses to exposure to Fulvestrant (CAS 129453-61-8). Zebrafish embryos were exposed to Fulvestrant according to OECD guidelines (OECD test No. 236). At the end of exposure time (96 hours), simultaneous RNA and protein extraction from 10 embryos was performed using a Macherey & Nagel RNA/protein extraction kit. The obtained RNA extracts were sequenced using Illumina NovaSeq 6000 system System and the obtained sequences went through bioinformatic analysis pipeline to Identify and count the detected gene sequences followed by differential gene expression analysis. Finally, potential substance specific biomarker candidates were refined and selected based on the differential expression patterns and the biological functions investigation of the detected DEGs.

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Sequencing of Danio rerio liver for 2 age groups

Project description:Comparison of Danio rerio liver for 2 age groups treated with Rotenone. The RNA-seq data comprisess two age groups treated with Rotenone. Jena Centre for Systems Biology of Ageing - JenAge (www.jenage.de)

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Sequencing of Danio rerio skin for 2 age groups

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