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RNAseq raw data of Salmonella Pullorum S06004

ABSTRACT: RNAseq raw data of Salmonella Pullorum S06004

PROVIDER: PRJEB76957 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	ERR13847258_1.fastq.gz	Fastqsanger.gz
	ERR13847258_2.fastq.gz	Fastqsanger.gz
	ERR13847259_1.fastq.gz	Fastqsanger.gz
	ERR13847259_2.fastq.gz	Fastqsanger.gz
	ERR13847260_1.fastq.gz	Fastqsanger.gz

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Expression profiles in different stages of the immune response to Salmonella enterica Pullorum in the chicken spleen

Project description:Salmonella enterica Pullorum（S. Pullorum） is one of the most important pathogens in poultry. A better understanding of the immune response and molecular modulation resulting from infection by S. Pullorum will facilitates the control of this pathogen. In this study, we determined the relationships among identified differential expressed genes (DEGs) and pathways via deeply mining microarray data from Guangxi Huang Chicken challenged with S. Pullorum.

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Prediction of Non-Coding sRNAs and Screening of Virulence-Related sRNAs of Salmonella Pullorum

Project description:Purpose: Searching for sRNAs in Salmonella pullorum by RNA sequencing and exploring their functions.Methods: High-throughput sequencing of RNA extracted from Salmonella pullorum under normal growth conditions to detect newly discovered sRNAs, followed by experiments to verify their functions.Results: The proportion of Clean Reads of this sequencing was >65%, and the base Q30s were all above 85%, indicating that the sequencing quality is good and can be used for subsequent analysis. The sRNAscanner software predicted that 148 new sRNAs might exist on the reference genome of Salmonella fowl dysentery, and the reads obtained from sequencing were compared to the genome, and it was found that 110 out of the 148 newly predicted sRNAs could be detected.Conclusions: sRNAs are widely found in bacteria and are involved in many physiological processes. In this study, we detected new sRNAs in Salmonella pullorum by RNA-seq, which lays the foundation for the subsequent investigation of the regulatory functions of sRNAs in bacteria.

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Expression profiles in different stages of the immune response to Salmonella enterica Pullorum in the chicken spleen

Project description:Salmonella enterica PullorumM-oM-<M-^HS. PullorumM-oM-<M-^I is one of the most important pathogens in poultry. A better understanding of the immune response and molecular modulation resulting from infection by S. Pullorum will facilitates the control of this pathogen. In this study, we determined the relationships among identified differential expressed genes (DEGs) and pathways via deeply mining microarray data from Guangxi Huang Chicken challenged with S. Pullorum. The chicks were then sacrificed via cervical dislocation immediately after anesthesia with 150 mg/kg sodium pentobarbital. This process was repeated at 2 hours post-infection (hpi), 4 hpi, 8 hpi, 24 hpi, 3 days post-infection (dpi), 5dpi, 7dpi, 12dpi, and 21dpi.The spleens were used to carrying out the microarray experiment. After total RNA extraction and quality control for each splenic sample were performed according to the standard protocol provided by Agilent Technologies, two random mRNA samples collected from the challenged group were equally mixed to hybridize with one chicken whole genome expression chip (Agilent.SingleColor.26441M-oM-<M-^I for each time point. In the challenged group, there were three biological replications (chips) for every time point. However, in the control group at each time point only one chip was used to hybridize with equally mixed mRNA sample containing the three control samples.

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Project description:The objectives of this study were to determine the protective effects of organic acids (OA) in broilers exposed to Salmonella Pullorum challenge at early stage and to explore the potential benefits of OA by metabolomics analysis. The treatment groups included non-challenged, S. Pullorum-challenged, challenged group supplemented with virginiamycin, challenged group supplemented with OA in drinking water, challenged group supplemented with OA in feed, and challenged group supplemented with OA in combination in drinking water and feed. Results showed that early Salmonella challenge induced an acute systemic infection of broilers in the starter phase, followed by the grower phase without triggering clinical signs. OA supplementation promoted growth during the grower phase, and while OA in water contributed more, the positive effects of OA in combination were comparable to those of virginiamycin supplementation in challenged birds. Furthermore, OA could modulate the systemic metabolic perturbation caused by challenge as it alleviated stress responses mediated by steroid hormone, potentially attenuated antioxidant or immune defense, and modified intestinal microbiota metabolism. These results show a metabolic mechanism that may partly explain the potential benefits of OA in Salmonella challenged birds, and may contribute to the use of OA to control or reduce S. Pullorum infection in farm animals.

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