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RNA-seq of Mycobacterium smegmatis MC2-155 with or without inducible overexpression of Mtb RpfB UTR with Atc

ABSTRACT: RNA-seq of Mycobacterium smegmatis MC2-155 with or without inducible overexpression of Mtb RpfB UTR with Atc

PROVIDER: PRJEB77850 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
	ERR13382816_1.fastq.gz	Fastqsanger.gz
	ERR13382816_2.fastq.gz	Fastqsanger.gz
	ERR13382817_1.fastq.gz	Fastqsanger.gz
	ERR13382817_2.fastq.gz	Fastqsanger.gz
	ERR13382818_1.fastq.gz	Fastqsanger.gz

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RNA-seq of Mycobacterium smegmatis MC2-155 with or without inducible overexpression of Mtb RpfB UTR with Atc

Project description:RpfB is one of five resuscitation promoting factors in Mycobacterium tuberculosis. We previously published data indicating the 5' UTR of the gene contains a regulatory riboswitch with an unknown (at the time) ligand. We overexpressed the switch in Mycobacterium smegmatis (which does not contain the rpfB gene), reasoning that overexpression of the switch would s sequester the unknown ligand, giving rise to a phenotype that would help us identify it.

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