Project description:We identified an enhancer element near IGF2 locus that is possibly involved with dopamine function and schizophrenia. A knockout mouse was generated for the enhancer element in the IGF2 locus. We then characterized the striatal synaptosomes ( i.e. biological fraction representing pre- post synaptic nerve terminal)by mass spectometry from WT and Igf2 enhancer KO mice.
Project description:We report the function of IGF2 in regulating the differentiation of ESCs, and then we performed RNA-seq for the wild-type and Igf2 knockout ESCs and embryoid bodies collected at different time points to analyze the transcriptomes. The results showed a significant difference between wild-type and Igf2 knockout groups in transcriptomes, and we performed a series experiments to study the function of Igf2 in the differentiation of ESCs.
Project description:To measure gene expression difference between wt and g9A knockout ES cells G9A TT2 ES cells (Yokochi et al) were treated with Veh. Or 4OHT (to delete G9A)
