Project description:Cassiopea andromeda is an ideal species for examining antioxidant stress response by non-calcifying zooxanthellate cnidarians because unlike its sister species Cassiopea xamachana, which is geographically restricted to Florida and the West Indies, C. andromeda typically occurs in tropical and subtropical waters worldwide. However, the molecular and cellular basis of antioxidant defences in Cassiopea species have largely been ignored but are fundamental to understanding the survival and distribution of these cnidarians, especially given that Cassiopea spp. themselves maybe useful indicators of marine ecosystem health in the face of increasing anthropogenic pollution and global climate change. Here we produced a high-quality draft genome sequence of C. andromeda that improves that will provide a foundational resource for advancing Cassiopea as a model research system for early diverging metazoans.
Project description:Cassiosomes are cell clusters that contain symbiotic dinoflagellates and nematocysts and released in a suspension of mucus by the up-side down jellyfish Cassiopea xamachana into the water column. Divers passing Cassiopea colonies experience skin irritation without directly disturbing the jellyfish. Cassiosomes and the structures that release them (vesicular appendages) were examined via shotgun mass spectrometry to determine whether these structures contain toxins that could be responsible for skin irritation.
Project description:Puccinia graminis f. sp. tritici is the cause of wheat stem rust. A microarray was designed from genes predicted from the P. graminis f. sp. tritici genome assembly, and gene expression measured for four conditions which include wheat or barley infecting growth stages initiated by urediniospores. mRNA was prepared from fresh urediniospores, uredinospores germinated for 24 hr, wheat seedlings infected with urediniospores for 8 days, and barley seedlings infected with urediniospores for 8 days. The asexual uredinial infection cycle on wheat produces additional urediniospores, which can start new cycles of wheat infection and are readily spread by aerial transport. This expression data is further described in Duplessis et al, Obligate Biotrophy Features Unraveled by the Genomic Analysis of the Rust Fungi, Melampsora larici-populina and Puccinia graminis f. sp. tritici