Project description:Mustard (Brassica juncea) was tested for Turnip mosaic virus infection. Small RNA of the plant was extracted and converted to DNA according to Ho, T., et al. (2006) Journal of Virological Methods 136:217-223, with primers modified to contain 454 adapter nucleotide sequences. The DNA then passed quality control through Bioanalyzer and Nanodrop before sequenced by 454 Life Sciences. Keywords: siRNA
Project description:LC-TOF-MS analysis of black mustard leaves exposed to methyl-jasmonate and caterpillar herbivory by Pieris brassicae. Metabolites: glucosinolates, phenylpropanoids (sinapic acid derivatives, and flavonol glucosides).
2019-08-08 | MSV000084183 | MassIVE
Project description:Brassica nigra genome sequencing and assembly
Project description:The endophytic fungi of certain grasses and herbaceous plants provide anti-herbivore defense compounds, thus living in mutualistic interaction with their hosts. Still, there is little information on such cooperation for tree-associated endophytes. We investigate the influence of the endophytic fungus Cladosporium cladosporioides on the chemical defenses of black poplar Populus nigra trees and the consequences on feeding preference, fitness of herbivorous insects, and insect community assembly. Strikingly, endophyte colonization increases both constitutive- and induced poplar defenses. Generalist Lymantria dispar larvae prefer and perform better on uninfected poplars due to the higher concentrations of salicinoids and fungal alkaloid stachydrine in endophyte-infected leaves. Under field conditions, the endophytic fungus shapes insect community assembly in young black poplar trees. Our results show that endophytic fungi can play a significant role in defending trees against herbivorous insects and structuring insect communities.
Project description:Brassica nigra plants, a Brassicaceae close to Arabidopsis thaliana, was used for combined stresses experiments. In this study, we performed a whole-genome microarray analysis on five-week-old plants and compared untreated plants and plants treated with ozone at 70 ppb, larvae of Pieris brassicae or both ozone followed by P. brassicae insect.
Project description:Oilseed mustard, Brassica juncea, exhibits high levels of genetic variability for salinity tolerance. To obtain the global view of transcriptome and investigate the molecular basis of salinity tolerance in a salt-tolerant variety CS52 of B. juncea, we performed transcriptome sequencing of control and salt-stressed seedlings. De novo assembly of 184 million high-quality paired-end reads yielded 42,327 unique transcripts longer than 300 bp with RPKM ≥1. When compared with non-redundant proteins, we could annotate 67% unigenes obtained in our study. Based on the mapping to expressed sequence tags (ESTs), 52.6% unigenes are novel compared to EST data available for B. juncea and constituent genomes. Differential expression analysis revealed altered expression of 1469 unigenes in response to salinity stress. Of these, 587, mainly associated with ROS detoxification, sulfur assimilation and calcium signaling pathways, are up regulated. Notable of these is RSA1 (SHORT ROOT IN SALT MEDIUM 1) INTERACTING TRANSCRIPTION FACTOR 1 (RITF1) homolog up regulated by >100 folds in response to stress. RITF1, encoding a bHLH transcription factor, is a positive regulator of SOS1 and several key genes involved in scavenging of salt stress-induced reactive oxygen species (ROS). Further, we performed comparative expression profiling of key genes implicated in ion homeostasis and sequestration (SOS1, SOS2, SOS3, ENH1, NHX1), calcium sensing pathway (RITF1) and ROS detoxification in contrasting cultivars, B. juncea and B. nigra, for salinity tolerance. The results revealed higher transcript accumulation of most of these genes in B. juncea var. CS52 compared to salt-sensitive cultivar even under normal growth conditions. Together, these findings reveal key pathways and signaling components that contribute to salinity tolerance in B. juncea var. CS52. We report transcriptome sequencing of two-weeks-old seedlings of B. juncea var. CS52 under normal growth conditions (CTRL) and in response to salinity stress (SS) using Illumina paired-end sequencing