Project description:Secrets of the hospital underbelly: abundance of antimicrobial resistance genes in hospital wastewater reflects hospital antimicrobial use and inpatient length of stay

| PRJEB34410 | ENA

Influence of a non-hospital medical care facility on antimicrobial resistance in wastewater.

Project description:The global widespread use of antimicrobials and accompanying increase in resistant bacterial strains is of major public health concern. Wastewater systems and wastewater treatment plants are considered a niche for antibiotic resistance genes (ARGs), with diverse microbial communities facilitating ARG transfer via mobile genetic element (MGE). In contrast to hospital sewage, wastewater from other health care facilities is still poorly investigated. At the instance of a nursing home located in south-west Germany, in the present study, shotgun metagenomics was used to investigate the impact on wastewater of samples collected up- and down-stream in different seasons. Microbial composition, ARGs and MGEs were analyzed using different annotation approaches with various databases, including Antibiotic Resistance Ontologies (ARO), integrons and plasmids. Our analysis identified seasonal differences in microbial communities and abundance of ARG and MGE between samples from different seasons. However, no obvious differences were detected between up- and downstream samples. The results suggest that, in contrast to hospitals, sewage from the nursing home does not have a major impact on ARG or MGE in wastewater, presumably due to much less intense antimicrobial usage. Possible limitations of metagenomic studies using high-throughput sequencing for detection of genes that seemingly confer antibiotic resistance are discussed.

| S-EPMC4379178 | biostudies-literature

hospital wastewater samples

Project description:hospital wastewater samples Metagenome

| PRJNA862690 | ENA

Transmission of antimicrobial resistance between hospital inpatients in a resource-limited setting

Project description:A description of the inter-patient antimicrobial resistance transmission within a tertiary hospital setting and investigating the relationship with physical separation on the ward.

| PRJEB39247 | ENA

Hospital sink wastewater

Project description:Metagenomic nanopore sequencing of hospital sink wastewater

| PRJNA1090773 | ENA