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HEP-TILE

ABSTRACT: Whole genome sequencing of hepatitis B virus (HBV) using tiled amplicon (HEP-TILE) and probe-based enrichment on Illumina and Nanopore platforms

PROVIDER: PRJEB79773 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR13935425_1.fastq.gz	Fastqsanger.gz
	ERR13935425_2.fastq.gz	Fastqsanger.gz
	ERR13935426_1.fastq.gz	Fastqsanger.gz
	ERR13935426_2.fastq.gz	Fastqsanger.gz
	ERR13935427_1.fastq.gz	Fastqsanger.gz

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HEP-TILE

Project description:Whole genome sequencing of hepatitis B virus (HBV) using tiled amplicon (HEP-TILE)

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Project description:The mammalian liver possesses a remarkable regenerative ability. 1) The 'oval cell' response emanates from the biliary tree when all hepatocytes are affected by chronic liver disease. 2) A massive, proliferative response of mature hepatocytes occurs upon acute liver damage such as partial hepatectomy (PHx). We establish a long-term 3D organoid culture system from mouse and human fetal/pediatric/adult hepatocytes that retain key morphological and functional features of hepatocytes fate. We report the mRNA and single cell sequencing of Hep-Orgs from different donors in different passages. We compared Hep-Orgs with primary hepatocytes, proliferative hepatocytes or Chol-Orgs derived from Epcam+ biliary cells. By analyzing, we determine similar gene expression profile of Hep-Orgs with primary hepatocytes and make genes lists distinguished with undamaged hepatocytes as well. We find the Hep-Orgs resemble proliferative damage-response of hepatocytes after partial hepatectomy while Chol-Orgs express high cholangiocytes markers. The sequencing data constitutes a valuable resource to understand liver organoids especially Hep-Orgs.

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