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Chalara RNA-Seq and variant discovery using mixed material collected from Norfolk, UK

ABSTRACT: RNA sequencing and variant discovery in the ash dieback pathogen (Hymenoscyphus fraxineus), using several infected samples collected from woodlands of Norfolk, UK

PROVIDER: PRJEB7998 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	foxley_wood1_vs_KW1-tgac.vcf.gz	Vcf
	wayland_wood1_vs_KW1-tgac.vcf	Other
	lolly_moor1_vs_KW1-tgac.vcf	Other
	ashwellthorpe1_vs_tgac1.vcf	Other
	ashwellthorpe2_vs_tgac1.vcf	Other

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Metabolomic profiling of Fraxinus excelsior genotypes tolerant or susceptible to ash dieback disease reveals changes in iridoid glycosides

Project description:European common ash, Fraxinus excelsior L., is currently threatened by Ash dieback (ADB) caused by the fungus, Hymenoscyphus fraxineus. To detect and identify metabolites that may be products of pathways important in contributing to resistance against H. fraxineus we performed untargeted metabolomic profiling on leaves from selected F. excelsior individuals showing strong tolerance or susceptibility to H. fraxineus. We identified sets of "features" [small molecules] that enabled strong discrimination between tolerant or susceptible genotypes of F. excelsior. Strikingly, tolerant F. excelsior lines exhibited low levels of iridoid glycosides, known anti-feeding deterrents. As Europe is threatened by Emerald Ash Borer (Agrilus planipennis), an invasive wood boring beetle native to East Asia that has devastated North American ash, our study raises the question whether selection for resistance to H. fraxineus leads to ecological trade-offs that result in susceptibility to emerging pests such as emerald ash borer.

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