Project description:In the past two decades, European ash trees (Fraxinus spp.) have been severely damaged due to ash dieback disease, which is caused by the fungal species Hymenoscyphus fraxineus (Chalara fraxinea in the anamorphic stage). Recent molecular phylogenetic and population genetic studies have suggested that this fungus has been introduced from Asia to Europe. During a fungal survey in Korea, H. fraxineus-like apothecia were collected from fallen leaves, rachises, and petioles of Korean ash and Manchurian ash trees. The morphological and ecological traits of these materials are described with the internal transcribed spacer rDNA sequence comparison of H. fraxineus strains collected from Korea, China and Japan.
Project description:Hymenoscyphus linearis isolate chi01 was grown on potato dextrose broth for 3 weeks. RNA was extracted from the mycelial mat and the library was prepared with TruSeq Stranded mRNA LT Sample Prep Kit and sequenced with HiSeq 2500 (2x100 bp).
Project description:Hymenoscyphus koreanus F52847-1 was grown on potato dextrose broth for 3 weeks. RNA was extracted from the mycelial mat and the library was prepared with TruSeq Stranded mRNA LT Sample Prep Kit and sequenced with HiSeq 2500 (2x100 bp).