Calendula officinalis
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB80524 | ENA |
REPOSITORIES: ENA
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Project description:Calendula officinalis cultivar:Rajskij sad Raw sequence reads
| PRJNA1021511 | ENA
Project description:The aim of the present study was to determine the effects of glucuronides of oleanolic acid (GlcUAOA) extracted from Calendula officinalis flower on the profile of immunogenic proteins of somatic extract of infective H. polygyrus bakeri L3 stage.
2021-04-08 | PXD024205 | Pride
Project description:Calendula officinalis isolate:TN-82-766 Genome sequencing and assembly
| PRJNA888267 | ENA
Project description:Regulus calendula (ruby-crowned kinglet), bRegCal1
| PRJNA1202539 | ENA
Project description:Regulus calendula (ruby-crowned kinglet) genomic and transcriptomic data
| PRJNA1202540 | ENA
Project description:Calendula officinalis L. is known as an ornamental plant as well as a source of biochemical compounds used in cosmetics and industry. C. officinalis has a complex karyotype. Published chromosome numbers differ between 2n = 4x = 28 or 32. We have estimated genome sizes in nine commercial cultivars and evaluated the ploidy level by karyotyping and fluorescent in situ hybridization (FISH) using 5S and 45S rDNA loci. The detection of chromosome sets of two rather than four homologues would suggest that C. officinalis has an allotetraploid background. In addition, four signals for 45S but only two for 5S were found by using FISH. Artificial chromosome doubling is a common technique in plant breeding, as polyploidization results in several consequences for plant growth and development. Especially the suggested allotetraploid background in C. officinalis is interesting when examining the effect of chromosome doubling on the plant phenotype. Here we describe chromosome doubling of three allotetraploid cultivars of C. officinalis, ‘Nova,’ ‘WUR 1553-7’ and ‘Orange Beauty’. Three antimitotic agents – colchicine, oryzalin and trifluralin - were used in different concentrations to find the combination of the best agent and the best dosage to obtain octaploids. For all three cultivars a few octaploids were obtained. A concentration of 200 and 400 ppm of colchicine was most efficient for chromosome doubling in ‘Nova’ and ‘Orange Beauty,’ respectively. For ‘WUR 1553-7’ the treatment with 20 ppm oryzalin was also effective. Cell numbers and first observations of the phenotype in the chromosome doubled plants show thicker leaves and bigger cells, as commonly observed after ploidy doubling. Due to the low number of chromosome doubled plants obtained more elaborate phenotyping will be performed on following generations cultivated under field conditions.
| S-EPMC7272713 | biostudies-literature
Project description:Premise of the studyFor the economically important species Calendula officinalis, a fast identification assay based on high-resolution melting curve analysis was designed. This assay was developed to distinguish C. officinalis from other species of the genus and other Asteraceae genera, and to detect C. officinalis as an adulterant of saffron samples.Methods and resultsFor this study, five markers (ITS, rbcL, 5' trnK-matK, psbA-trnH, trnL-trnF) of 10 Calendula species were sequenced and analyzed for species-specific mutations. With the application of two developed primer pairs located in the trnK 5' intron and trnL-trnF, C. officinalis could be distinguished from other species of the genus and all outgroup samples tested. Adulterations of Calendula DNA in saffron could be detected down to 0.01%.ConclusionsWith the developed assay, C. officinalis can be reliably identified and admixtures of this species as adulterant of saffron can be revealed at low levels.
| S-EPMC4651632 | biostudies-literature