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The effect of oleanolic acid glucuronides from marigold (Calendula officinalis) on the possible modification of immunogenic proteins from infective Heigmosomoides polygyrus bakeri larvae (L3)

ABSTRACT: The aim of the present study was to determine the effects of glucuronides of oleanolic acid (GlcUAOA) extracted from Calendula officinalis flower on the profile of immunogenic proteins of somatic extract of infective H. polygyrus bakeri L3 stage.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Heligmosomoides Polygyrus Bakeri

SUBMITTER: Ludmiła Szewczak

LAB HEAD: Maria Doligalska

PROVIDER: PXD024205 | Pride | 2021-04-08

REPOSITORIES: pride

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Calendula officinalis Triterpenoid Saponins Impact the Immune Recognition of Proteins in Parasitic Nematodes.

Doligalska Maria M Jóźwicka Kinga K Szewczak Ludmiła L Nowakowska Julita J Brodaczewska Klaudia K Goździk Katarzyna K Pączkowski Cezary C Szakiel Anna A

Pathogens (Basel, Switzerland) 20210304 3

The influence of triterpenoid saponins on subcellular morphological changes in the cells of parasitic nematodes remains poorly understood. Our study examines the effect of oleanolic acid glucuronides from marigold (Calendula officinalis) on the possible modification of immunogenic proteins from infective Heligmosomoides polygyrus bakeri larvae (L3). Our findings indicate that the triterpenoid saponins alter the subcellular morphology of the larvae and prevent recognition of nematod ...[more]

PMID: 33806494

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Project description:BACKGROUND: Differences between noninfective first-stage (L1) and infective third-stage (L3i) larvae of parasitic nematode Strongyloides stercoralisat the molecular level are relatively uncharacterized. DNA microarrays were developed and utilized for this purpose. METHODS AND FINDINGS: Oligonucleotide hybridization probes for the array were designed to bind 3571 putative mRNA transcripts predicted by analysis of 11,335 expressed sequence tags (ESTs) obtained as part of the Nematode EST project. RNA obtained from S. stercoralis L3i and L1 was co-hybridized to each array after labeling the individual samples with different fluorescent tags. Bioinformatic predictions of gene function were developed using a novel cDNA Annotation System software. We identified 967 differentially expressed genes (457 L3i-biased; 510 L1-biased) having greater than two-fold expression differences and p < 0.01. Based on functional analysis, L1s have a larger number of genes putatively involved in transcription (p = 0.0158), and L3is have higher expression of stress-related genes (such as putative heat shock proteins dnj-12, daf-21, dnj-10). Genes with products that have been shown to be immunoreactive in S. sterocoralis-infected humans (SsIR and NIE) were additionally found to be L3i biased. Unique and abundant L3i contigs of interest included S. stercoralis orthologs of cytochrome oxidase ucr 2.1, daf-12, and daf-21, which may be potential chemotherapeutic targets. The S. stercoralis ortholog of fatty acid and retinol binding protein-1, successfully used in a vaccine against Ancylostoma ceylanicum, was identified among the top 25 most L3i-biased genes. The sperm-containing glycoprotein domain, utilized in a vaccine against the nematode Cooperia punctata, was exclusively found in the L3i group and may be a valuable S. stercoralis target of interest. Conclusions A new DNA microarray tool for the examination of S. stercoralis biology has been developed and provides new and valuable insights regarding differences between infective and noninfective S. stercoralis larvae. Potential therapeutic and vaccine targets were identified for further study.

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Dataset Information

The effect of oleanolic acid glucuronides from marigold (Calendula officinalis) on the possible modification of immunogenic proteins from infective Heigmosomoides polygyrus bakeri larvae (L3)

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Publications

<i>Calendula officinalis</i> Triterpenoid Saponins Impact the Immune Recognition of Proteins in Parasitic Nematodes.

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