Project description:To determine 3D chromosomal structure in differentating ectoderm of the frog Xenopus laevis, we performed tethered conformation capture (TCC) (PMID: 22198700) on wild-type dissected ectoderm and ectoderm injected with multicilin. We used these data to investigate possible differences between these conditions (we found very few) and also to determine the positions of topologically-associated domains (TADs). These data were also used to facilitate chromosome-level assembly of the X. laevis genome.
Project description:Intermediate filaments called lamins line the metazoan nuclear envelope and organize the nucleus and genome. Unlike actin and microtubules, purified intermediate filament proteins assemble into non-physiological structures, making it difficult to connect lamin functions to their assembly and regulation. To overcome this challenge, we reconstituted lamin assembly without nuclear assembly using interphase Xenopus laevis egg extracts that recapitulate physiological context. Mimicking nucleoplasm conditions triggers dispersed lamin assembly in egg extracts. Such ectopic lamin assembly occurs on nuclear pore complex-containing membranes, but does not recruit known nuclear lamina components, demonstrating that lamin assembly is separable from the rest of the nuclear lamina and nucleus. This assembly assay in the physiological context of cellular components opens the door to mechanistically dissecting nuclear lamina function in nuclear organization.
2026-09-16 | PXD067048 | Pride
Project description:Integrated morphological and molecular approaches of Entoloma aprile on Ulmus laevis
Project description:RNA sequencing (RNA-seq) experiment was performed on 18 samples of Nitzschia laevis cells collected at 3 h, 6 h and 12 h after inoculation in treated medium with glucose addition (+G) and control group (-G) respectively. The de novo assembly totally generated 16650 unigenes.