Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effects of HIST1H2ac knockdown on the DNA methylation landscape of breast cancer cell line MCF-7

ABSTRACT: Effects of HIST1H2ac knockdown on the DNA methylation landscape of breast cancer cell line MCF-7

PROVIDER: PRJEB8564 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR760653_1.fastq.gz	Fastqsanger.gz
	ERR760653_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 2 of 2

Similar Datasets

Effects of HIST1H2ac knockdown on the DNA methylation landscape of breast cancer cell line MCF-7

Project description:There are 64 replication-dependent histone genes with different isotypes associated with each class of the five histone types, H1, H2a, H2b, H3 and H4 in the human genome. In a previous report, we have showed that HIST1H2ac serves as a master regulator of ERalpha-dependent gene activation via ERalpha recruitment, and mediates chromatin looping of regulatory elements of estrogen receptor-targeted genes. Here, we performed whole genome bisulfite sequencing of MCF-7 upon suppressing H2ac expression through siRNA transfection using two different siRNAs to determine the knockdown effect on DNA methylation. Our analysis showed the DNA methylation of ~99% of the genome were mostly unchanged comparing to normal MCF-7 (methylation levels difference within 0.2).

2020-12-31 | E-MTAB-3320 | biostudies-arrayexpress

Histone methyltransferase SMYD3 knockdown effects in MCF7 breast cancer cell line

Project description:The study aims to elucidate the effect of histone methyltransferase SMYD3 on gene expression in MCF-7 breast cancer cell line. Knockdown luciferase control v.s. knockdown SMYD3 in MCF-7 breast cancer cell line were conducted. Results identify a large proportion of cell cycle-related genes regulated by SMYD3.

2016-11-17 | GSE58048 | GEO

RNA-seq of MCF-7 (breast adenocarcinoma) and 2102Ep (embryonic carcinoma) cells upon LINE-1 knockdown.

Project description:To measure the levels of expression of retrotransposon individual copies, and particularly of LINE-1 (L1) elements of the L1HS-Ta subfamily, we performed 2x150 bp paired-end and strand-specific RNA-seq on polyA+ RNA of MCF-7 and 2102Ep cells, which express high levels of L1. To confirm the origin of the L1-specific signal, we also performed RNA-seq upon shRNA-mediated L1 knockdown. sh960 relates to a scramble shRNA control. sh1083 and sh1085 relate to two distinct shRNAs directed against the ORF1 region of L1.3 (GenBank L19088) as a prototype L1HS-Ta.

2016-05-25 | E-MTAB-3788 | biostudies-arrayexpress

INPP4B over-expressed INPP4B MCF-7 luminal breast cancer cell line

Project description:INPP4B over-expressed INPP4B MCF-7 luminal breast cancer cell line

2020-06-01 | MSV000085526 | MassIVE

CHIP knockdown effect on breast cancer cell line

Project description:Analysis of MCF-7 cell clones lacking the E3 ubiquitin liganse CHIP. We used microarrays to detail the global gene expression profiles effected by CHIP in human brest cancer. Total RNA was extracted from CHIP or LacZ knockdown MCF7 and hybridized on Affymetrix microarrays.

2016-07-25 | E-GEOD-49349 | biostudies-arrayexpress

Estrogen response in breast cancer cell line MCF-7 is dependent on NR2F2 [RNA-seq]

Project description:We show that most binding events of NR2F2 occur together with the ERα binding sites.To address the functional relationship between NR2F2 and ERα, we assessed the role of NR2F2 in oestrogen-induced growth in ER positive cell line MCF-7. The MTT experiment showed that inhibition of NR2F2 prevented the oestrogen-induced proliferation of MCF-7 cells.To further explore the effect of NR2F2 on estrogen response, We expanded our knockdown studies by performing RNA-seq analysis for MCF-7 cells transfected with control or NR2F2 shRNAs with or without E2.

2019-10-15 | GSE132433 | GEO

ZNF703 overexpression and/or knockdown in the breast cancer cell line MCF-7

Project description:The telomeric amplicon at 8p12 is common in ER+ breast cancers. Array-CGH and expression analyses of 1172 tumors revealed ZNF703/Zeppo1 was the single gene within the minimal amplicon and was amplified predominantly in the Luminal B subtype. Amplification was shown to correlate with increased gene and protein expression and was associated with a distinct expression signature and poor outcome. In the luminal MCF-7 cell line manipulation of ZNF703 expression altered transcription of genes also present within the primary tumor signature, including TGFBR2 (whose promoter was bound by ZNF703). Overexpression of ZNF703 rendered MCF-7 cells insensitive to TGFβ-induced suppression of mammosphere formation. Forced overexpression of ZNF703 in normal human breast epithelial cells enhanced the frequency of in vitro colony-forming cells from luminal progenitors. Together these data strongly point to ZNF703/Zeppo1 as a novel oncogene in Luminal B breast cancer.

2011-03-27 | GSE23673 | GEO

ZNF703 overexpression and/or knockdown in the breast cancer cell line MCF-7

2011-03-26 | E-GEOD-23673 | biostudies-arrayexpress

miRNA expression in Twist overexpressing breast cancer cell line MCF-7

Project description:The breast cancer cell line MCF-7 was engineered to overexpress the Twist gene resulting in the MCF-7/Twist cell line. To study which miRNA are regulated by Twist, we employed whole genome microarray expression profiling and compared miRNA expression between MCF-7/Twist and MCF-7 cells.

2023-06-03 | GSE151773 | GEO

Expression analysis upon SIRT1 knockdown in MCF-7 cells

Project description:SIRT1 is a nuclear NAD+-dependent protein deacetylase. Expression microarray analysis was used to study the effect of SIRT1 knockdown on gene expression in MCF-7 breast cancer cells.

2009-05-28 | GSE13459 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data