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Role of Ccr4-Not Complex in Heterochromatin Formation at Meiotic Genes and Subtelomeres in Fission Yeast (VII)

ABSTRACT: Role of Ccr4-Not Complex in Heterochromatin Formation at Meiotic Genes and Subtelomeres in Fission Yeast (VII)

PROVIDER: PRJEB9087 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR852064.fastq.gz	Fastqsanger.gz
	ERR852065.fastq.gz	Fastqsanger.gz
	ERR852066.fastq.gz	Fastqsanger.gz
	ERR852067.fastq.gz	Fastqsanger.gz
	ERR852068.fastq.gz	Fastqsanger.gz

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Role of Ccr4-Not Complex in Heterochromatin Formation at Meiotic Genes and Subtelomeres in Fission Yeast (VII)

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Expression profiling of fission yeast strains: wild-type, erh1∆, and ccr4∆

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Role of the Ccr4/Not complex in the regulation of heterochromatin in Schizosaccharomyces pombe

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Role of the Ccr4/Not complex in the regulation of heterochromatin in Schizosaccharomyces pombe (II)

Project description:RIP-chip experiments to identify RNAs associated with components of the Ccr4-Not complex in fission yeast

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Role of the Ccr4/Not complex in the regulation of heterochromatin in Schizosaccharomyces pombe (VI)

Project description:RIP-chip experiments to identify RNAs associated with components of the Ccr4-Not complex in fission yeast

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Role of the Ccr4/Not complex in the regulation of heterochromatin in Schizosaccharomyces pombe (VII)

Project description:Expression profiling of mutants predicted to abolish the catalytic activity of Ccr4

2015-09-25 | E-MTAB-3493 | biostudies-arrayexpress

Role of Ccr4-Not complex in heterochromatin formation at meiotic genes and subtelomeres in fission yeast.

Project description:BackgroundHeterochromatin is essential for chromosome segregation, gene silencing and genome integrity. The fission yeast Schizosaccharomyces pombe contains heterochromatin at centromeres, subtelomeres, and mating type genes, as well as at small islands of meiotic genes dispersed across the genome. This heterochromatin is generated by partially redundant mechanisms, including the production of small interfering RNAs (siRNAs) that are incorporated into the RITS protein complex (RNAi-Induced Transcriptional Silencing). The assembly of heterochromatin islands requires the function of the RNA-binding protein Mmi1, which recruits RITS to its mRNA targets and to heterochromatin islands. In addition, Mmi1 directs its targets to an exosome-dependent RNA elimination pathway.ResultsCcr4-Not is a conserved multiprotein complex that regulates gene expression at multiple levels, including RNA degradation and translation. We show here that Ccr4-Not is recruited by Mmi1 to its RNA targets. Surprisingly, Ccr4 and Caf1 (the mRNA deadenylase catalytic subunits of the Ccr4-Not complex) are not necessary for the degradation or translation of Mmi1 RNA targets, but are essential for heterochromatin integrity at Mmi1-dependent islands and, independently of Mmi1, at subtelomeric regions. Both roles require the deadenylase activity of Ccr4 and the Mot2/Not4 protein, a ubiquitin ligase that is also part of the complex. Genetic evidence shows that Ccr4-mediated silencing is essential for normal cell growth, indicating that this novel regulation is physiologically relevant. Moreover, Ccr4 interacts with components of the RITS complex in a Mmi1-independent manner.ConclusionsTaken together, our results demonstrate that the Ccr4-Not complex is required for heterochromatin integrity in both Mmi1-dependent and Mmi1-independent pathways.

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Meiotic cohesins modulate chromosome compaction during meiotic prophase in fission yeast

Project description:The meiotic cohesin Rec8 is required for the stepwise segregation of chromosomes during the two rounds of meiotic division. By directly measuring chromosome compaction in living cells of the fission yeast Schizosaccharomyces pombe, we found an additional role for the meiotic cohesin in the compaction of chromosomes during meiotic prophase. In the absence of Rec8, chromosomes were decompacted relative to those of wild-type cells. Conversely, loss of the cohesin-associated protein Pds5 resulted in hyper-compaction. While this hyper-compaction requires Rec8, binding of Rec8 to chromatin was reduced in the absence of Pds5, indicating that Pds5 promotes chromosome association of Rec8. To explain these observations, we propose that meiotic prophase chromosomes are organized as chromatin loops emanating from a Rec8-containing axis; the absence of Rec8 disrupts the axis, resulting in disorganized chromosomes, whereas reduced Rec8 loading results in a longitudinally compacted axis with fewer attachment points and longer chromatin loops. Keywords: ChIP-chip analysis

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Expression profiling of fission yeast strains: wild-type, erh1â??, and ccr4â??

Project description:We report gene expression profiling in the fission yeast Schizosaccharomyces pombe. We performed high-throughput sequencing of RNA isolated from wild-type, erh1â??, and ccr4â?? strains. We find that many meiotic gene containing degradation sequence DSR are expressed in vegetative erh1â??, while these meiotic mRNAs do not increase in ccr4â??, indicating that Erh1 and Ccr4 target different set of genes during vegetative growth. RNA sequencing in wild-type, erh1â?? and ccr4â?? strains. Two biological replicates for erh1â?? and ccr4â??, and one sample for wild-type because we already reported wild-type data previously.

2016-06-15 | E-GEOD-76112 | biostudies-arrayexpress

Meiotic cohesins modulate chromosome compaction during meiotic prophase in fission yeast

2010-07-01 | E-GEOD-5284 | biostudies-arrayexpress