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GENOME-WIDE STUDY OF STRUCTURAL VARIANTS IN BOVINE HOLSTEIN, MONTBELIARDE AND NORMANDE DAIRY BREEDS

ABSTRACT: Deep whole genome sequencing of 62 bulls representing the three major dairy breeds in France was performed in order to investigate chromosomal alterations between individual samples across the genome.

PROVIDER: PRJEB9343 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR883660_1.fastq.gz	Fastqsanger.gz
	ERR883660_2.fastq.gz	Fastqsanger.gz
	ERR883661_1.fastq.gz	Fastqsanger.gz
	ERR883661_2.fastq.gz	Fastqsanger.gz
	ERR883662_1.fastq.gz	Fastqsanger.gz

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Project description:Milk is a highly nutritious natural product that provides not only a rich source of amino acids to the consumer but also hundreds of bioactive peptides and proteins known to elicit health-benefitting activities. We investigated the milk protein profile produced by Holstein and Jersey dairy cows maintained under the same diet, management and environmental conditions using proteomic approaches that optimize protein extraction and characterization of the low abundance proteins within the skim milk fraction of bovine milk. In total, 935 low abundance proteins were identified. Gene ontology classified all proteins identified into various cellular localization and function categories. A total of 43 low abundance proteins were differentially expressed between the two dairy breeds. Bioactive proteins involved in host-defense, including lactotransferrin (P=0.0026) and complement C2 protein (P=0.0001), were differentially expressed by the two breeds, whereas others such as osteopontin (P=0.1788) and lactoperoxidase (P=0.2973) were not. This work is the first to outline the protein profile produced by two important breeds of dairy cattle maintained under the same diet, environment and management conditions in order to observe likely true breed differences. This research now allows us to better understand and contrast further research examining the bovine proteome that includes these different breeds.Within the last decade, the amount of research characterizing the bovine milk proteome has increased due to growing interest in the bioactive proteins that are present in milk. Proteomic analysis of low abundance whey proteins has mainly focused on human breast milk; however, previous research has highlighted the presence of bioactive proteins in bovine milk. Recent publications outlining the cross-reactivity of bovine bioactive proteins on human biological function highlight the need for further investigation into the bovine milk proteome. The rationale behind this study is to characterize and compare the low abundance protein profile in the skim milk fraction produced from Holstein and Jersey breeds of dairy cattle, which are two major dairy cattle breeds in the USA. A combination of fractionation strategies was used to efficiently enrich the low abundance proteins from bovine skim milk for proteomic profiling. A total of 935 low abundance proteins were identified and compared between the two bovine breeds. The results from this study provide insight into breed differences and similarities in the milk proteome profile produced by two breeds of dairy cattle.

Dataset Information

GENOME-WIDE STUDY OF STRUCTURAL VARIANTS IN BOVINE HOLSTEIN, MONTBELIARDE AND NORMANDE DAIRY BREEDS

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