Project description:We performed Illumina sequencing of sRNA libraries prepared from 10-day-old seedlings in Arabidopsis. SE is a key component in miRNA processing and pre-mRNA splicing, whereas ATXR5/6 is engaged in epigenetic silencing. Genome-wide sRNA sequencing revealed that ATXR5/6 did not participate in SE-mediated miRNA biogenesis.
Project description:Total RNA extracted from 15 knocking down treated 293T cells using siRNAs targeting transcription splicing factors and sequenced using Illumina Hiseq PE150 platform to generate RNA sequencing with 150bp in read length. Nearly 50 million raw reads were yielded from each sample respectively. We used FastQC to confirm the quality of raw fastq sequencing data, and Ericscript software to detect fusion transcripts.
