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Genome-wide identification of transcriptional targets for H2AK119ub and H3K36me2 in LP-1 control and PSMD14 knockdown cells

ABSTRACT: Genome-wide identification of transcriptional targets for H2AK119ub and H3K36me2 in LP-1 control and PSMD14 knockdown cells

PROVIDER: PRJNA1001908 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25510008_1.fastq.gz	Fastqsanger.gz
	SRR25510008_2.fastq.gz	Fastqsanger.gz
	SRR25510009_1.fastq.gz	Fastqsanger.gz
	SRR25510009_2.fastq.gz	Fastqsanger.gz
	SRR25510010_1.fastq.gz	Fastqsanger.gz

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Genome-wide identification of transcriptional targets for H2AK119ub and H3K36me2 in LP-1 control and PSMD14 knockdown cells

Project description:To investigate the PSMD14-NSD2 complex track with H2AK119ub or H3K36me2, CUT&Tag was performed in LP-1 cells infected with lentiviruses carrying control shRNA or PSMD14 shRNA using antibodies against H2AK119ub or H3K36me2. Following CUT&Tag, DNAs were amplified using non-biased conditions, labeled, and sequenced with Illumina NovaSeq 150PE.

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Genome-wide identification of transcriptional targets for PSMD14 and NSD2 in LP-1 cells

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H3K36me2 ChIP-chip

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Project description:Polycomb repressive complexes( PRCs) play critical role in cell fate decisions during normal development as well as disease progression through mediate histone modifications such as H3K27me3 and H2AK119ub. How exactly PRCs recruited to chromatin remains to be fully illuminated. Here, we report that YTHDF1, a N6-methyladenine (m6A) RNA reader previously known being mainly cytoplasmic, associates with RNF2, a PRC1 protein that mediates H2AK119ub in human embryonic stem cells (hESCs). Specifically, a portion of YTHDF1 localizes in the nuclei and associates with RNF2/ H2AK119ub on a subset of gene loci related to neural development functions. Knock-down YTHDF1 attenuates H2AK119ub modification on these genes and promotes neural differentiation in hESCs. Our findings provide another non-canonical mechanism of YTHDF1 to participate in PRC1 mediated chromatin modification in hESCs.

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YTHDF1 facilitates PRC1 mediated H2AK119ub in Human ES Cells

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Project description:Genome-wide identification of transcriptional targets for PSMD14 and NSD2 in LP-1 cells

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