Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1002752 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR25554948_1.fastq.gz | Fastqsanger.gz | |||
| SRR25554948_2.fastq.gz | Fastqsanger.gz | |||
| SRR25554949_1.fastq.gz | Fastqsanger.gz | |||
| SRR25554949_2.fastq.gz | Fastqsanger.gz | |||
| SRR25554950_1.fastq.gz | Fastqsanger.gz |
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Project description:Background. Transforming waste and non-food materials into bulk biofuels and chemicals represents a major stride in creating a sustainable bioindustry, optimizing the use of resources while reducing environmental footprints. Yet, despite these advancements, the production of high-value natural products often continues to rely on first-generation substrates, underscoring the intricate processes and specific requirements of their biosynthesis. This is also true for Streptomyces lividans, a renowned host organism celebrated for its capacity to produce and uncover a wide array of natural products, attributed to its genetic versatility and potent secondary metabolism. Given this context, it becomes imperative to assess and optimize this microorganism for the synthesis of natural products specifically from waste and non-food substrates. Results. We metabolically engineered S. lividans TK24 to heterologously produce the ribosomally synthesized and post-translationally modified peptide, bottromycin, as well as the polyketide, pamamycin. The modified strains successfully produced these compounds using waste and non-food model substrates like protocatechuate (derived from lignin), 4-hydroxybenzoate (sourced from plastic waste), and mannitol (from seaweed). Comprehensive transcriptomic and metabolomic analyses offered insights into how these substrates influenced the cellular metabolism of S. lividans. When evaluating production efficiency, S. lividans showcased remarkable tolerance, especially in a fed-batch process using a mineral medium containing the toxic aromatic 4-hydroxybenzoate, leading to enhanced and highly selective bottromycin production. Additionally, it generated a unique spectrum of pamamycins when cultured in mannitol-rich seaweed extract without the need for added nutrients. Conclusion. Our study showcases the successful production of high-value natural products using varied waste and non-food raw materials, thereby circumventing the reliance on costly, food-competing resources. S. lividans exhibited remarkable adaptability and resilience across these diverse substrates. When cultured on aromatic compounds, it displayed a distinct array of intracellular CoA esters, presenting promising avenues for polyketide production. Future research could focus on enhancing S. lividans' substrate utilization pathways to more efficiently process the intricate mixtures commonly found in waste and non-food sources.
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Project description:Zebrafish (n=420; 70/treatment) were weighed and placed in 8L aerated tanks,<br>served with flow-through soft-water at 25ml/min. A combination of Mariotte bottles<br>were used to dose tanks with Cu (concentrated Cu solution made from CuSO4 dissolved<br>in 0.05% HNO3), Na (concentrated NaCl solution), and Ca (concentrated CaCl2 solution)<br>to 6 treatment regimes of either control (Ctrl), Cu only (Cu), high Ca only (Ca), Ca + Cu<br>(CaCu), high Na only (Na), and Na + Cu (NaCu; See Table 1 for ion concentrations).<br>Nitric acid had no measurable impact on water pH. Fish were fed 2% body weight of<br>commercial tropical fish food, once per day. Tanks were monitored daily for mortality<br>and cleaned of any food or waste that had accumulated. Water samples (10 ml) were<br>taken from each tank, filtered though a 0.45?m filtration disc (Pall Corporation, East<br>Hills, NY), added to a plastic tube containing 100?l HNO3 and kept at 4oC for analysis of<br>ion content and Cu concentration. Throughout the experiment, there were no mortalities<br>in any of the tanks. At the end of the exposure period, fish were quickly euthanized by<br>overdose of buffered aesthetic (MS-222, Sigma) and sampled for gill, liver, and gut,<br>which were immediately frozen in liquid N2 for further analysis of Cu burden, gene<br>expression, and enzyme activity.
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Project description:Microbial community structures in microbial electrolysis cells (MECs) fed with waste activated sludge (WAS)
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