Transcription profiling of Danio rerio livers following exposure to combinations of copper, sodium and calcium
Ontology highlight
ABSTRACT:
served with flow-through soft-water at 25ml/min. A combination of Mariotte bottles
were used to dose tanks with Cu (concentrated Cu solution made from CuSO4 dissolved
in 0.05% HNO3), Na (concentrated NaCl solution), and Ca (concentrated CaCl2 solution)
to 6 treatment regimes of either control (Ctrl), Cu only (Cu), high Ca only (Ca), Ca + Cu
(CaCu), high Na only (Na), and Na + Cu (NaCu; See Table 1 for ion concentrations).
Nitric acid had no measurable impact on water pH. Fish were fed 2% body weight of
commercial tropical fish food, once per day. Tanks were monitored daily for mortality
and cleaned of any food or waste that had accumulated. Water samples (10 ml) were
taken from each tank, filtered though a 0.45?m filtration disc (Pall Corporation, East
Hills, NY), added to a plastic tube containing 100?l HNO3 and kept at 4oC for analysis of
ion content and Cu concentration. Throughout the experiment, there were no mortalities
in any of the tanks. At the end of the exposure period, fish were quickly euthanized by
overdose of buffered aesthetic (MS-222, Sigma) and sampled for gill, liver, and gut,
which were immediately frozen in liquid N2 for further analysis of Cu burden, gene
expression, and enzyme activity.
ORGANISM(S): Danio rerio
SUBMITTER: Paul Craig
PROVIDER: E-MEXP-2288 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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