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Isolation, identification and osteogenic capability analysis of mesenchymal stem cells derived from different layers of human maxillary sinus membrane

ABSTRACT: Isolation, identification and osteogenic capability analysis of mesenchymal stem cells derived from different layers of human maxillary sinus membrane

PROVIDER: PRJNA1004538 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25609224_1.fastq.gz	Fastqsanger.gz
	SRR25609224_2.fastq.gz	Fastqsanger.gz
	SRR25609225_1.fastq.gz	Fastqsanger.gz
	SRR25609225_2.fastq.gz	Fastqsanger.gz
	SRR25609226_1.fastq.gz	Fastqsanger.gz

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Isolation, identification and osteogenic capability analysis of mesenchymal stem cells derived from different layers of human maxillary sinus membrane

Project description:Mesenchymal stem cells (MSCs) from different tissue sources have different biological processes and differentiation abilities. Although previous studies confirm that MSCs derived from human maxillary sinus membrane (hMSM) can be cultured in vitro, it cannot confirm whether they belong to the same type and possess the same differentiation ability. In this study, lamina propria layer derived MSCs (hLMSCs) and periosteum layer derived MSCs (hPMSCs) from hMSM are isolated and expanded using explant cell culture method and the biological characteristics of hLMSCs and hPMSCs are compared using RNA sequencing. According to RNA sequencing and subsequent functional verification, hLMSCs and hPMSCs are identified as CD171+/CD90+ and CD171-/CD90+ phenotypes separately. This study suggests that two different types of MSCs can be identified and isolated from hMSMs. Both cells serve as good MSCs potential candidates for bone regeneration. It also indicates that hLMSCs and hPMSCs are valuable in vitro tools for preclinical studies and future exploration of the origin osteogenesis machenism in maxillary sinus region.

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Promoting effect of rapamycin on osteogenic differentiation of maxillary sinus membrane stem cells.

Project description:BackgroundStem cells located in the maxillary sinus membrane can differentiate into osteocytes. Our study aimed to evaluate the effect of rapamycin (RAPA) on the osteogenic differentiation of maxillary sinus membrane stem cells (MSMSCs).MethodsColony-forming unit assay, immunophenotype identification assay, and multi-differentiation assay confirmed characteristics of MSMSCs obtained from SD rats. Transmission electron microscopy (TEM) and flow cytometry (FCM) identified the initial autophagic level of MSMSCs induced by RAPA. Real-time quantitative PCR (qPCR) evaluated subsequent autophagic levels and osteogenic differentiation. Alkaline phosphatase (ALP) activity assay and alizarin red staining (ARS) evaluated subsequent osteogenic differentiation. We performed a histological examination to clarify in vivo osteogenesis with ectopic bone mass from BALB/c nude mice.ResultsMSMSCs possessed an active proliferation and multi-differentiation capacity, showing a phenotype of mesenchymal stem cells. The autophagic level increased with increasing RAPA (0, 10, 100, 1,000 nM) and decreased over time. ALP activity and calcium nodules forming in four RAPA-treated groups on three-time points (7, 14, 21 d) showed significant differences. Col1a1, Runx2, and Spp1 expressed most in 100 nM RAPA group on 7 and 14 d. Osteogenesis-related genes except for Ibsp expression between four groups tended to be consistent on 21 d. 100 nM and 10 nM RAPA-treated groups showed more bone formation in vivo.ConclusionRAPA can promote osteogenic differentiation of MSMSCs, indicating a possible relationship between osteogenic differentiation and autophagy.

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Project description:mouse maxillary sinus augmentation research

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Transcriptome analysis of osteogenic differentiation of human maxillary sinus mesenchymal stem cells using RNA-Seq

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Comparison of DNA methyltransferase-1 (Dnmt1) deleted urogenital sinus epithelium compared to wildtype urogenital sinus epithelium

Project description:Dnmt1 is an important regulator of tissue development and differentiation. To assess the effects of epithelium Dnmt1 deletion in the developing urogenital sinus (precursor of the urethra and prostate in males), we isolated urogenital sinus epithelial tissue from Dnmt1 deleted mouse embryos and wildtype mouse embryos. The transcriptomes were analyzed by RNA-seq

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Alectinib Induces Sinus Bradycardia through Inhibiting Cacna1d Expression, Mediating Electro-Dysfunction in Sinus Node

Project description:Alectinib is the first-line therapy for anaplastic lymphoma kinase rearranged non-small cell lung cancer. Sinus node bradycardia as a major adverse cardiac event still widely impact patient’s quality of life. However, the underlying mechanism of alectinib induce sinus bradycardia (AISB) remains elusive. The aim of this study was to reveal the pathogenesis of the AISB in a rat model including the electrophysiology alterations and the molecular basis

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